酶联免疫试剂盒（ELISA Kit）

武汉华美具有完善的ELISA试剂盒开发平台，成熟的抗原、抗体研发系统，熟练掌握各种酶联技术，如双抗夹心法、（直接）竞争法、间接竞争法、阻断法、间接法、双抗原夹心法等方法，结合我公司的诊断试剂开发团队我们可以有效的将试剂盒开发为临床诊断级别，质量处于世界前列。

我们的ELISA试剂盒，采用三轮质检方式，最大限度地保证了试剂盒最小的批内差、批间差。试剂盒组分经过重重优化，每个组分的稳定性都完美的经过高温破坏测验，最大程度的避免了长途运输和保存过程中抗原抗体的失活，武汉华美试剂盒凭借其完美的标曲、严格的质控、较高的稳定性，在世界范围内的科研试剂领域中脱颖而出，深受数十个国家上万名使用者的青睐。

• 别名:
  VegfcVascular endothelial growth factor C ELISA kit; VEGF-C ELISA kit; Flt4 ligand ELISA kit; Flt4-L ELISA kit; Vascular endothelial growth factor-related protein ELISA kit; VRP ELISA kit
• 
  种属:Mus musculus (Mouse)
• Homo sapiens (Human)Rattus norvegicus (Rat)
• 待测物名称:
  vascular endothelial growth factor C
• 缩写:
  VEGFC
• 蛋白功能1:
  Angiogenesis
• 蛋白功能3:
  Angiogenesis
• 检测范围:
  1.56 pg/mL-100 pg/mL
• 灵敏度:
  0.39 pg/mL
• 反应时间:
  1-5h
• 所需样本体积:
  50-100ul
• 检测波长:
  450 nm
• 
  精密度:标准曲线:

  These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

  pg/ml OD1 OD2 Average Corrected 100 2.600 2.511 2.556 2.462 50 2.020 2.152 2.086 1.992 25 1.391 1.366 1.379 1.285 12.5 0.735 0.744 0.740 0.646 6.25 0.331 0.321 0.326 0.232 3.12 0.255 0.235 0.245 0.151 1.56 0.188 0.186 0.187 0.093 0 0.097 0.091 0.094

• Intra-assay Precision (Precision within an assay): CV%<8%
  Three samples of known concentration were tested twenty times on one plate to assess.
  Inter-assay Precision (Precision between assays): CV%<10%
  Three samples of known concentration were tested in twenty assays to assess.

• 线性度:

  To assess the linearity of the assay, samples were spiked with high concentrations of mouse VEGF-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  		Sample		Serum(n=4)
  1:1		Average %		85
  		Range %		80-92
  1:2		Average %		95
  		Range %		91-105
  1:4		Average %		102
  		Range %		92-110
  1:8		Average %		90
  		Range %		86-98

• 回收率:

  The recovery of mouse VEGF-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
  Sample Type		Average % Recovery		Range
  Serum (n=5)		94		89-98
  EDTA plasma (n=4)		96		90-100

• 定性/定量:
  quantitative
• 研究领域:
  Cancer
• 引用文献:

  1. VEGF-C-driven lymphatic drainage enables immunosurveillance of brain tumours , Song E,Nature,2020

  2. Inhibition of host NOX1 blocks tumor growth and enhances checkpoint inhibitor-based immunotherapy , Stalin J, et al,Life Science Alliance,2019

  3. VEGF-C promotes the development of lymphatics in bone and bone loss , Devon Hominick.et al,Elife. 2018,2018

  4. Heterogeneity in VEGFR3 levels drives lymphatic vessel hyperplasia through cell-autonomous and non-cell-autonomous mechanisms , Yan Zhang.et al,NAT COMMUN,2018

  5. Interactions between fibroblastic reticular cells and B cells promote mesenteric lymph node lymphangiogenesis , Kumar Dubey L.et al,Nat Commun.,2017

  6. In vivo and in vitro effect of hepatocarcinoma lymph node metastasis by upregulation of Annexin A7 and relevant mechanisms , Wang XY. et al,Tumour Biol,2015

  7. Adipose-Derived Stem Cells Promote Proliferation, Migration, and Tube Formation of Lymphatic Endothelial Cells In Vitro by Secreting Lymphangiogenic Factors , Takeda K et al,Ann Plast Surg,2014

  8. Therapeutic Lymphangiogenesis With Implantation of Adipose-derived Regenerative Cells , Shimizu Y et al,J Am Heart Assoc(Journal of the American Heart Association (JAHA) ),2012