This Human IL-4 Ready-SET-Go! ELISA Kit (including pre-coated ELISA plates) contains the necessary reagents, buffers and diluents for performing quantitative enzyme linked immunosorbent assays (ELISA). This ELISA kit is specifically engineered for accurate and precise measurement of human IL-4 protein levels from samples including serum, plasma, and supernatants from cell cultures.

Components

1. Pre-coated ELISA Plate, clone 8D4-8
2. Detection Antibody: Pre-titrated, biotin-conjugated antibody, clone MP4-25D2
3. Standard: Recombinant cytokine for generating standard curve and calibrating samples
4. Assay Diluent: 5X concentrated
5. ELISA Wash Buffer Powder
6. Detection enzyme: pre-titrated Avidin-HRP
7. Substrate Solution: Tetramethylbenzidine (TMB) Substrate Solution
8. Stop Solution: 5 mls of 1X solution per plate
9. Certificate of Analysis: Lot-specific instructions for dilution of antibodies and standards