产品描述

Category Name： Infectious Diseases

Method: ELISA

Principle: ELISA

Controls Standards: Positive, Negative and Calibrator

Sensitivity: Not available

Sample: 100 µl

Runtime： 50 Minutes

Wavelength： 450 nm

Safety： Please refer to MSDS

Shelflife： 18 Months

INTENDED USE

The Calbiotech EBV-VCA IgA ELISA Kit is intended for the detection of IgA antibody to EBV-VCA in human serum or plasma.

SUMMARY AND EXPLANATION

Epstein-Barr virus (EBV) is a herpes virus known to cause infectious mononucleosis (IM). EBV infection may demonstrate a wide spectrum of clinical symptoms. The majorities of primary EBV infections are transmitted via saliva, occur during childhood, and are sub-clinical. In the U.S., 50% of the population demonstrate EBV antibodies before the age of 5 years; 80% by adulthood. Transfusion-associated EBV infections have also been reported. Epstein-Barr virus has also been associated in the pathogenisis of two human cancers, Burkitt's lymphoma and nasopharyngeal carcinoma. Burkitt's lymphoma is primarily observed in Sub-Sahara Africa, especially in African children, and in New Guinea. Nasopharyngeal carcinoma is observed in Asia, most notably in Southern China.

PRINCIPLE OF THE TEST

Diluted patient serum is added to wells coated with purified antigen. IgA specific antibody, if present, binds to the antigen. All unbound materials are washed away and the enzyme conjugate is added to bind to the antibody-antigen complex, if present. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the hydrolysis of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgA specific antibody in the sample.