Skeletal muscle cells are one of the largest cells in the body. They are multinucleate formed by the fusion of myoblasts. Skeletal muscle regeneration is a complex process in which many factors are involved. When skeletal muscle suffers an injury, quiescent resident myoblasts called satellite cells are activated to proliferate, migrate, and finally differentiate. Various cellular signaling pathways, such as phosphatidylinositol 3-kinase, calcineurin, Janus kinase 2/signal transducer and activator of transcription 3 (STAT3), and mitogen-activated protein kinase (MAPK) have been suggested to play an important role in skeletal muscle growth. RI-stimulated glucose transport in cultured human skeletal muscle is mediated by GLUT4 and heparan sulfate proteoglycan is involved in skeletal muscle differentiation. The fusion of mononucleated cells to form multinucleated myotubes is a central event in skeletal muscle development. Controlling the onset and progression of this process is a complex set of interactions between myoblasts and their environment. Skeletal muscle cell culture is a useful model for studying this process of cell differentiation.HSkMC from AcceGen Research Laboratories are isolated from human muscle of the pectoral girdle. HSkMC are cryopreserved at primary culture and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HSkMC are characterized by immunofluorescent method with antibodies to myosin, actin and actinin. HSkMC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HSkMC are guaranteed to further expand for 15 population doublings at the conditions provided by AcceGen Research Laboratories.