Screen Quest 发光法钙离子检测试剂盒

¥4356
AAT Bioquest
进口
2021-09-02 10:12

上海齐源生物科技有限公司

我要认领
上海齐源生物科技有限公司
杨阳
15021410950
3123991360@qq.com
产品属性
数量大量
英文名Screen Quest™ Luminometric Calcium Assay Kit
CAS号/
保质期详见说明
供应商齐源生物
保存条件详见说明
规格10 Plates
产品说明
齐源生物专业代理美国AAT Bioquest产品,并有大量储存现货欢迎致电:021-59588506 
关键词:Screen Quest 发光法钙离子检测试剂盒详细如下
Calcium flux assays are preferred methods in drug discovery for screening G protein coupled receptors (GPCR). This kit uses a highly calcium-sensitive and membrane-permeable coelenterazine analog as a calcium indicator for the cells that are transfected with apoaequorin gene. Aequorin is a calcium-sensitive bioluminescent protein from the jellyfish Aequorea victoria that has been used extensively as a calcium indicator in cells. The aequorin complex emits blue light when bound to calcium ions. The luminescence intensity is directly proportional to the Ca2+ concentration. Our coelenterazine-based kit is much more sensitive than the fluorescence-based calcium assay kits (such as Fluo-4, Fluo-3, Calcium-3 and Calcium-4). This kit provides an optimized assay method for monitoring G-protein-coupled receptors (GPCRs) and calcium channels. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation. It might be useful for monitoring of intracellular calcium mobilization in a specified compartment given that recombinant apoaequorin proteins can now be targeted to specific organelles, cells and tissues.
Components
Component A: Coelenterazine Analog 1 vial, lyophilized
Component B: 100% ETOH 1 vial (500 µL)
Component C: Assay Buffer 1 bottle (100 mL-1X ready to use)
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