Anti-β-catenin Rabbit Polyclonal Antibody

¥1600
NewEastBio
美国
2021-09-02 15:21

武汉纽斯特生物技术有限公司

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武汉纽斯特生物技术有限公司
乐先生
15071267082 027-87561033
neweastbio@126.com
929192314
产品属性
抗体名β-catenin
抗体英文名β-catenin Rabbit Polyclonal Antibody
靶点CTNNB1, CTNNB
浓度1 mg/ml
应用范围ELISA, WB, IF, IHC
宿主Rabbit
适应物种脊椎动物
保质期一年
抗原来源Rabbit
目录编号21282
级别实验级
数量30000
供应商NewEastBio
标记物FITC
克隆性单克隆
保存条件-20℃
形态液体
亚型IgG
免疫原A synthetic peptide from the internal region of β-catenin
规格100μl
产品说明
β-catenin [21282]
[100 µL]
Catalog Number: 21282
Gene Symbol: CTNNB1, CTNNB
Description: Anti-β-catenin Rabbit Polyclonal Antibody
Background: Beta-catenin (or β-catenin) is a protein that in humans is encoded by the CTNNB1 gene. β-catenin is a subunit of the cadherin protein complex and also acts as an intracellular signal transducer in the Wnt signaling pathway. Deregulation of beta-catenin signaling is an important event in the genesis of a number of malignancies, such as colon cancer, melanoma, hepatocellular carcinoma, ovarian cancer, endometrial cancer, medulloblastoma pilomatricomas, and prostate cancer.
Immunogen:  A synthetic peptide from the internal region of β-catenin.
Applications: ELISA, WB, IF, IHC
Recommended Dilutions:
ELISA: 1:1000-1:5000 
WB: 1:500-1:2000 
IHC: 1:50-1:100
Concentration: 1 mg/ml
Host: Rabbit
Format: Liquid
Clonality: Polyclonal
Isotype:
Purity:  Purified from serum
Storage buffer:
Preservative: no
Constituents: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Recognizes β-catenin from vertebrates.
Storage Conditions:  Store at -20°C. Avoid freeze / thaw cycles
Western blot:
Western blot analysis of recombinant β-catenin protein. Purified His-tagged β-catenin protein (amino acids 1-76) was blotted with anti- β-catenin rabbit polyclonal antibody (Cat. #21282).
Immunohistochemistry:
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with anti-β-catenin polyclonal antibody (Cat. #21282). Tissue samples were fixed with formaldehyde and blocked with 1% serum for 15 min at 37 °C. Antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were then incubated with primary antibody (1:50 dilution) overnight at 4°C. A HRP-conjugated Goat anti-mouse IgG (1:50 dilution) was used as secondary antibody.