品牌:CST，产品名称：Ras Antibody #3965
英文名称：Ras Antibody
Product name：Ras Antibody #3965
Source：Rabbit
Sensitivity：Endogenous
MW(kDa)：21
Applications：WB ,
Purification： Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues close to the amino-terminus of human K-Ras. Antibodies are purified by protein A and peptide affinity chromatography.
Background： The 21 kDa guanine-nucleotide binding proteins (K-Ras, H-Ras, and N-Ras) cycle between active (GTP-bound) and inactive (GDP-bound) forms (1). Receptor tyrosine kinases and G protein-coupled receptors activate Ras, which then stimulates the Raf-MEK-MAPK pathway (2-4). GTPase-activating proteins (GAP) normally facilitate the inactivation of Ras. However, research studies have shown that in 30% of human tumors, point mutations in Ras prevent the GAP-mediated inhibition of this pathway (5). The most common oncogenic Ras mutation found in tumors is Gly12 to Asp12 (G12D), which prevents Ras inactivation, possibly by increasing the overall rigidity of the protein (5,6).

Entrez-Gene Id

Swiss-Prot Acc.

Specificity / Sensitivity:

Ras Antibody detects endogenous levels of total K-Ras, H-Ras, and N-Ras. This antibody may also cross-react with R-Ras and M-Ras.

Species Reactivity:

Human, Mouse, Rat, Monkey, D. melanogaster, Pig, S. cerevisiae

Species predicted to react based on 100% sequence homology:

Hamster, Chicken, Xenopus, Zebrafish, Dog

Storage:

Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.