产品货号:jlcR7462
英文名称:ATG14
中文名称:自噬相关蛋白14抗体
别名:4832427M01; ATG14; Atg14L; Autophagy-related protein 14-like protein; BAKOR_HUMAN; Barkor; Beclin 1-associated autophagy-related key regulator; D14Ertd114e; D14Ertd436e; KIAA0831; mCG_6911.
研究领域:细胞生物信号转导细胞凋亡转运蛋白
抗体来源:Rabbit
克隆类型 : Polyclonal
交叉反应: Human, Mouse, Rat, Dog, Pig, Cow,
产品应用:WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分子量 :55kDa
细胞定位:细胞浆
性状 :Lyophilized or Liquid
浓度:1mg/ml
免疫原:KLH conjugated synthetic peptide derived from human ATG14:41-140/492
亚型:IgG
纯化方法:affinity purified by Protein A
储存液 : 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件:Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
PubMed:PubMed
产品介绍:Required for both basal and inducible autophagy. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner.
Function:
Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex. Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine. Promotes BECN1 translocation from the trans-Golgi network to autophagosomes. Enhances PIK3C3 activity in a BECN1-dependent manner.
Subunit:
Component of the autophagy-specific PI3-kinase complex I composed of ATG14, BECN1, PIK3C3 and PIK3R4, but not UVRAG, nor KIAA0226/Rubicon. UVRAG and ATG14/Barkor form mutually exclusive complexes with BECN1 through direct competition. The complex containing ATG14 up-regulates autophagy, while the one containing Rubicon down-regulates autophagy (By similarity). Interacts with PIK3CB (By similarity). Interacts with BECN1P1/BECN2.
Subcellular Location:
Cytoplasm. Endoplasmic reticulum. Cytosolic under nutrient-rich conditions. Following autophagy stimuli, such as starvation or rapamycin induction, predominantly detected in cytoplasmic foci, identified as isolation membranes and autophagosomes.
Similarity:
Belongs to the Barkor family.
SWISS:
Q6ZNE5
Gene ID:
22863
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
