Buccutite PE-Cy7抗体标记试剂盒 标记100ug抗体

¥1
AAT Bioquest
进口
2021-09-06 10:27

上海齐源生物科技有限公司

我要认领
上海齐源生物科技有限公司
杨阳
15021410950
3123991360@qq.com
产品属性
数量大量
英文名Buccutite™ Rapid PE-Cy7 Tandem Antibody Labeling Kit
CAS号/
保质期详见说明
供应商齐源生物
保存条件详见说明
规格2 Labelings
产品说明
齐源生物专业代理美国AAT Bioquest产品,并有大量储存现货欢迎致电:021-59588506 
关键词:Buccutite PE-Cy7抗体标记试剂盒 标记100ug抗体详细如下
PE-Cy7 is a popular color used in flow cytometry. Its primary absorption peak is at 565 nm with emission peak at ~780 nm. AAT Bioquest offers this Buccutite™ rapid labeling kit to facilitate the PE-Cy7 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Buccutite™ PE-Cy7 Conjugation Kit provides a robust and convenient method to conjugate your antibodies with PE. The kit includes an activated PE and reaction buffer. The conjugated antibody can be used in flow cytometry, WB, ELISA and IHC applications. This kit is sufficient for 2 labeling reactions, each up to 100 ug of antibody. Considering the large size of PE (240 kDa), the amount of antibody used in a labeling reaction must always be less than the amount of RPE. The best ratio for any new antibody reagent must be determined by experimentation but 50-60 ug of IgG antibody for every 100 ug of RPE usually gives optimal results. Our kit provides preactivated PE-Cy7 to facilitate the PE-Cy7 tandem conjugations to antibodies and other proteins such as streptavidin and other secondary reagents. Our preactivated PE-Cy7 tandem is ready to conjugate, giving much higher yield than the conventionally tedious SMCC-based conjugation chemistry. In addition, our preactivated PE-Cy7 tandem is conjugated to a protein via its amino group that is abundant in proteins while SMCC chemistry targets the thiol group that has to be regenerated by the reduction of antibodies.
Component A: Buccutite™ FOL-Activated PE-Cy7 2 vials (lyophilized)
Component B: Buccutite™ MTA 2 vials (lyophilized)
Component C: Reaction Buffer 1 vial (20 µL)
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