Amplite 胆固醇定量试剂盒

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AAT Bioquest
进口
2021-09-06 12:52

上海齐源生物科技有限公司

我要认领
上海齐源生物科技有限公司
杨阳
15021410950
3123991360@qq.com
产品属性
数量大量
英文名Amplite™ Cholesterol Quantitation Kit
CAS号/
保质期详见说明
供应商齐源生物
保存条件详见说明
规格200 Tests
产品说明
齐源生物专业代理美国AAT Bioquest产品,并有大量储存现货欢迎致电:021-59588506 
关键词:Amplite 胆固醇定量试剂盒详细如下
Cholesterol is required to build and maintain cell membranes. It modulates membrane fluidity over the range of physiological temperatures. Within cells, cholesterol is the precursor molecule in several biochemical pathways. Cholesterol is also an important precursor molecule for the synthesis of Vitamin D and the steroid hormones, including the adrenal gland hormones cortisol and aldosterone as well as the sex hormones progesterone, estrogens, together with testosterone and their derivatives. This Amplite™ Cholesterol Quantitation Assay Kit provides one of the most sensitive methods for quantifying cholesterol. The kit uses Amplite™ Red to quantify the concentration of cholesterol, which is related to the production of hydrogen peroxide in the cholesterol oxidase-mediated enzyme coupling reactions in the presence of cholesterol. The amount of cholesterol is proportional to the concentration of hydrogen peroxide formed in the enzyme coupling reaction cycle. In the presence of peroxidase, the fluorescence intensity of Amplite™ Red is proportional to the concentration of hydrogen peroxide that is converted to the concentration of cholesterol. The assay can be readily read with a fluorescence microplate reader.
Components
Component A: Amplite™ Red (light sensitive) 1 vial
Component B: Assay Buffer 1 bottle (20 mL)
Component C: Cholesterol Enzyme Mix (lyophilized) 2 bottles
Component D: Cholesterol Standard 1vial (2 mM, 100 µL)
Component E: DMSO 1 vial (200 µL)
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