Originally developed in Professor Austin Smith's laboratory, NDiff™ Neuro-2 Medium Supplement is a serum-free, N2-like supplement for the in vitro differentiation of murine embryonic stem (ES) cells into post-mitotic neurons particularly via monolayer differentiation. This product may also be used in the derivation, propagation and maintenance of mouse NS neural stem cells.
Key Applications:
Cell Culture
Stem Cell Culture
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Cell Culture Media or Reagent Type:
Reagents & Supplements
Stem Cell Type:
Neural Stem Cells
Stem Cell Workflow Stage:
Maintenance & Expansion
Differentiation
Media Type:
Cell Culture Media Additives
Storage Conditions:
Maintain at -20 °C until expiration date. Protect from light. Once thawed, aliquot unused portion into smaller volumes and store at -20 °C until future use. Once added to the cell culture medium, the product is stable at 4 °C for four weeks.
Cell Culture Reagent Type:
Cell Culture Supplements
Formulation:
NDiff Neuro-2 Medium Supplement contains Insulin, Apo-Transferrin, Sodium Selenite, Putrescine, and Progesterone. This media supplement is supplied as a 200X stock and should be added to the cell culture medium at a final concentration of 1X.
