产品名称 ：
ADP:ATP比值测试盒 | EnzyLight™ ADP/ATP Ratio Assay Kit

分类名称 :
代谢产物分析

产品介绍 :
测定方法 快速生物发光法测定组织或细胞中ADP：ATP的比例。检测时间：20分钟。运输：冷运。储存：-20°C。

Description Changes in the ADP/ATP ratio have been used to differentiate modes of cell death and viability. Increased levels of ATP and decreased levels of ADP signify proliferating cells. Conversely, decreased levels of ATP and increased levels of ADP represent apoptotic or necrotic cells where the decrease in ATP and increase in ADP is much more pronounced in necrosis versus apoptosis. our EnzyLight^TM ADP/ATP Ratio Assay Kit provides a rapid method to measure ADP and ATP levels for the screening of apoptosis, necrosis and cell proliferation in mammalian cells. The assay involves two steps. In the first step, the working reagent lyses cells to release ATP and ADP. In the presence of luciferase, ATP immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration.In the second step, the ADP is converted to ATP through an enzyme reaction. This newly formed ATP then reacts with the D-luciferin as in the first step. Due to a special formulation of the reagent system which greatly stabilizes the light signal generated by the luciferase reaction, the luminescence from the initial ATP measurement remains stable throughout this assay. Therefore, the second light intensity measured represents the total ADP and ATP concentration in the sample.This non-radioactive, homogeneous cell-based assay is performed in microplates. The reagent is compatible with all culture media and with all liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.

Key features Safe. Non-radioactive assay.Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash and reagent transfer steps are involved.Robust and amenable to HTS: Z’ factors of 0.5 and above are routinely observed in 96-well and 384-well plates. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.

Applications Apoptosis and Necrosis determination in cells.Cell proliferation: effects of cytokines, growth factor, nutrients.Drug discovery: high-throughput screening for anticancer drugs.

Storage conditions: store all reagents at -20°C. This product is shipped on dry ice. Shelf life of at least 6 months.

其他 :
编号：ELDT-048，ELDT-100
测试：48T,100T

公司简介：
博世生物提供优质、快速(如2分钟)、方便的生化检测试剂盒，广泛适用于人和动物等生物样品、食品和环境监测样品中相关指标的检测。博世专注于无害、无放 射性的检测技术(如吸收光、荧光、生物化学发光法等)，安全可靠，适合各类仪器(分光光度仪、微孔读数仪等）。产品具有快速、简单、高通量（可用于HTS 药物筛选）、灵敏(特别适合小鼠样品) 、准确性高和低干扰等主要特征。检测步骤简便，用户不需花额外的时间来作调适测试。
博世生物产品主要包括：1、血液尿液化学；2、酶活性测定；3、能量代谢；4、氧化应激；5、高通量HTS试剂；6、阴阳离子检测。