Anti-Hepatitis C Virus Core Antigen antibody (mAb C1F5)
Product overview
| Description | Mouse monoclonal C1F5to Hepatitis C Virus Core Antigen |
| Host species | Mouse |
| Tested applications | ELISA, IF, WB |
| Immunogen | Purified HCV core fusion protein (genotype 1b). |
| Epitope | This antibody recognizes an epitope between amino acid residues 21-40 of HCV core protein. This sequence is conserved among different HCV genotypes. |
Properties| Form | Liquid |
| Storage instructions | Store at 4°C for short period or -20°C for long term. |
| Storage buffer | PBS |
| Clonality | Monoclonal |
| Clone number | C1F5 |
| Isotype | IgG1 |
Applications| ELISA | ELISA: Use at an assay dependent dilution.
|
| IF | IF: Use at an assay dependent dilution. Staining of HCV core protein in transfected cells, HCV infected cell cultures or tissues, which yields a staining pattern consistent with cytoplasmic and vesicular staining. |
| WB | WB: 1:1000
Use a concentration of 1µg/ml. (This antibody detects a single ~21 kDa protein representing HCV core protein in transfected cell lines or HCV cell cultures. |
Relevance Hepatitis C Virus is a positive, single stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins and several non structural proteins necessary for viral replication. Hepatitis C virus (HCV) causes most cases of non-A, non-B hepatitis and results in most HCV infected people developing chronic infections, liver cirrhosis and hepatocellular carcinoma. T cell responses, including interferon-gamma production are severely suppressed in chronic HCV patients.
The hepatitis C virus (HCV) core protein represents the first 191 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. Hepatitis C virus (HCV) core is a viral structural protein; it also participates in some cellular processes, including transcriptional regulation. However the mechanisms of core-mediated transcriptional regulation remain poorly understood.
Hepatitis C virus (HCV) core protein is thought to contribute to HCV pathogenesis through its interaction with various signal transduction pathways. In addition, HCV core antigen is a recently developed marker of hepatitis C infection. The HCV core protein has been previously shown to circulate in the bloodstream of HCV-infected patients and inhibit host immunity through an interaction with gC1qR.
