Mouse basic fibroblast growth factor ELISA Kit

96 Tests

Operating instruction

FOR RESEARCH USE ONLY!

Alternative name

Mouse BFGF ELISA KIT ,Mouse basic fibroblast growth factor ELISA KIT ,Mouse FGF-2 ELISA KIT ,Mouse FGF2 ELISA KIT ,Mouse basic fibroblast growth factor bFGF ELISA KIT ,Mouse FGFB ELISA KIT ,Mouse HBGF-2 ELISA KIT ,Mouse fibroblast growth factor 2 (basic) ELISA KIT ,Mouse heparin-binding growth factor 2 ELISA KIT ,Mouse prostatropin ELISA KIT ,Mouse fibroblast growth factor 2 ELISA KIT ,Mouse Heparin-binding growth factor 2 ELISA KIT ,Mouse HBGF-2 ELISA KIT

Intended use

Mouse BFGF ELISA KIT allows for the in vitro quantitative determination of Mouse basic fibroblast growth factor concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids.

Test principle

The microtiter plate provided in Mouse BFGF ELISA KIT has been pre-coated with an antibody specific to BFGF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for BFGF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain BFGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of BFGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Range 15.6-1000pg/ml

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