Components
This product is a mixture of glycopeptides antibiotics containing Bleomycin A2 (70%) and B2(30%). Bleomycins differ from each other in the terminal amine and show varying biological activity.
Biochem/physiol Actions
Mode of Action: Bleomycin Sulfate binds to DNA, inhibits DNA synthesis, and causes single strand DNA scission at specific base sequences. This antibiotic can also cleave RNA, to a lesser degree but more selectively, and ats as an inducer and regulator of apoptosis and inhibits tumor angiogenesis. To cleave DNA, it is required to bind oxygen and a metal ion.
Caution
This product should be stored at 2-8°C.
包装
Packaged in a bottomless glass bottle with its contents inside an inserted fused cone.
Preparation Note
The product is soluble in water in water at 20 mg/mL and is active for several days. A solution of 2 units/mL in 0.1 M neutral potassium phosphate should be used within 14 days. A solution is active for three months at 3 units/mL in normal saline.
Application
Bleomycin sulfate is used to study tumor-specific cytotoxic activity in certain cell lines such as human normal oral cells(gingival fibrobast HGF, pulp cell HPC and periodontal ligament fibroblast HPLF), human oral squamous cell carcinoma (HSC-2, HSC-3, HSC-4, Ca9-22 and NA) and human promyelocytic leukemia HL-60 cells1. It is used to study free radical-mediated mechanisms of DNA strand scission/breaking and as a transformed cell selection agent, especially for plant transformants. Recommended for use as a selection agent at 10-100 μg/ml.
