| 数量 | 现货 |
| 供应商 | 博士德生物 |
| 检测限 | 156pg/ml-10000pg/ml |
| 检测方法 | 酶联免疫双抗夹心法 |
| 应用 | cell culture supernates, serum and plasma(heparin, EDTA). |
| 适应物种 | Mouse |
| 规格 | 48T/96T |
| 货号 | EK0371 |
|---|---|
| 产品名称 | Mouse ICAM-1 PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of mouse ICAM-1 in cell culture supernates, serum and plasma(heparin, EDTA). |
| 免疫原 | NSO, Q28-N485 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 156pg/ml-10000pg/ml |
| 基因名称 | ICAM1 |
| 基因全名 | intercellular adhesion molecule 1 |
| Uniprot ID | P13597 |
| 指标别名 | BB2|CD 54|CD54|CD54 antigen|Cell surface glycoprotein P3.58|Human rhinovirus receptor|ICAM 1|Intercellular adhesion molecule 1|Major group rhinovirus receptor|MALA2|MGC6195|MyD10|P3.58|Surface antigen of activated B cells |
| 背景资料 | Intercellular adhesion molecule-1(ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated(LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1(ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen(LFA-1) or macrophage-1 antigen(Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses. The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds. The single chain is Gln28-Asn 485. It has 457 amino acids with the molecular mass of 76.8kDa. As a result of glycosylation, the molecular mass of 110-130KDa is revealed by SDS-PAGE. |
| 显色时间 | 20-25 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK0371-CAP | 96-well plate precoated with anti- mouse ICAM-1 antibody | 1 |
| EK0371-ST | Lyophilized recombinant mouse ICAM-1 standard | 10ng/tube×2 |
| EK0371-DA | Biotinylated anti- mouse ICAM-1 antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 354 | 1378 | 4978 | 361 | 1458 | 5187 |
| Standard deviation | 21.94 | 62.01 | 318.59 | 25.27 | 83.1 | 414.96 |
| CV(%) | 6.2% | 4.5% | 6.4% | 7% | 5.7% | 8% |
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.059 | 0.124 | 0.129 | 0.297 | 0.451 | 0.640 | 1.345 | 1.916 |
