产品概况
| 产品名称 | TCP1 beta(CCT2) Antibody |
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| 产品描述 | Anti-TCP1 beta(CCT2) Antibody validated for WB, ELISA in Human. |
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| 文献引用格式 | TCP1 beta(CCT2) Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM1665) |
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| 应用范围 | WB, ELISA |
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| 抗体来源 | Mouse |
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| 抗体亚型 | IgG1 |
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| 免疫原 | Purified recombinant fragment of human CCT2 expressed in E. Coli. |
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| 抗体规格 | 200ul/1180元 |
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产品详情
| 克隆 | Monoclonal |
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| 克隆号 | 单克隆抗体:IgG1;克隆号:5B5F5 |
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| 产品形态 | Liquid |
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| 内容 | Ascitic fluid containing 0.03% sodium azide. |
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| 储存条件 | 连续使用时4℃存储,保质期六个月;长期存储时建议分装为10ul以上小包装-20℃存储,并避免反复冻融,保质期一年。 |
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指标相关信息 (来源: Uniprot.org)
小贴士:你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
| 基因名 | CCT2 |
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| 基因全名 | T-complex protein 1 subunit beta |
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| Tissue Specificity | Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. . |
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| Sequence Similarities | T-complex protein 1 subunit beta;TCP-1-beta;CCT-beta;CCT2;99D8.1, CCTB; |
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| Uniprot ID | CCT2: P78371 |
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指标背景简介
The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Two transcript variants encoding different isoforms have been found for this gene.
产品应用细节
为了提供最优质的抗体,博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测,以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
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| Western blotting | 1:100-400 |
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| Immunohistochemistry in paraffin section | 1:50-200 |
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| Immunocytochemistry in fixed cells | 1:50-200 |
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| Flow Cytometry | 1:40-80 |
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| enzyme-linked immuno sorbent assay | 1:2000 |
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*最优稀释度需要用户自己调试,此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片
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[list_product_images]CCT2(BM1665)(MW:54KD)Hela,MCF7,Jurkat细胞裂解,免疫印迹分析。[/list_product_images]