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| 数量 | 现货 |
| 供应商 | 博士德生物 |
| 检测限 | 31.2pg/ml-2000pg/ml |
| 检测方法 | 酶联免疫双抗夹心法 |
| 应用 | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA, citrate). |
| 适应物种 | 酶联免疫双抗夹心法 |
| 规格 | 96T |
| 货号 | EK0308-CN |
|---|---|
| 产品名称 | Dog Canine BDNF PicoKine™ ELISA Kit |
| 产品描述 | Sandwich High Sensitivity ELISA kit for Quantitative Detection of Dog Canine BDNF. 96wells/kit, with removable strips. |
| 免疫原 | Expression system for standard: sf21; Immunogen sequence: H129-R247 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <15pg/ml |
| 检测范围 | 31.2pg/ml-2000pg/ml |
| 基因名称 | Bdnf |
| 基因全名 | Brain-derived neurotrophic factor |
| Uniprot ID | P23363 |
| 指标别名 | Brain-derived neurotrophic factor;BDNF;Bdnf; |
| 背景资料 | Brain-derived neurotrophic factor(BDNF) is a prosurvival factor induced by cortical neurons that is necessary for survival of striatal neurons in the brain. It is a secreted protein with the molecular weight of 27.8kDa, consisting of 247 amino acids. It is known to promote neuronal survival and differentiation. BDNF shares substantial amino acid sequence identity with nerve growth factor(NGF). BDNF and neurotrophin-3(NT-3) are two recently cloned neurotrophic factors that are homologous to NGF. mRNA products of the BDNF and NT-3 genes are detected in the adult human brain, suggesting that these proteins are involved in the maintenance of the adult nervous system. BDNF and other neurotrophins are critically involved in long-term potentiation(LTP). BDNF-mediated LTP is induced postsynaptically. BDNF has trophic effects on serotonergic(5-HT) neurons in the central nervous system. BDNF has an essential maintenance function in the regulation of anxiety-related behavior and in food intake through central mediators in both the basal and fasted state. It plays a role in treating breathing disorders such as respiratory insufficiency after spinal injury. |
| 显色时间 | 20-25 |
| SKU | 名称 | 数量 |
| Intra-Assay Precision | Inter-Assay Precision |
| n="3">I | ||||||
| ntra-Assay Precisio | ||||||
| n | ||||||
| n="3">I | ||||||
| nter-Assay Precisio | ||||||
| n | ||||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 109 | 447 | 11121 | 121 | 468 | 1257 |
| Standard deviation | 3.60 | 17.43 | 51.39 | 8.35 | 33.23 | 90.5 |
| CV(%) | 3.3 | 3.9 | 4.3 | 6.9 | 7.1 | 7.2 |
| Concentration(pg/ml) | 0 | 2000 | 2000 | 2000 | 2000 | 2000 | 2000 | 2000 |
| O.D. | 0.063 | 0.095 | 0.133 | 0.216 | 0.358 | 0.720 | 1.405 | 2.298 |
