本公司提供科研ER-Alpha雌激素受体α抗体（用于western blot），抗体质量可靠，订购ER-Alpha雌激素受体α抗体（用于western blot）请联系在线客服或者销售人员。
抗体参数如下>>>>
中文名称：雌激素受体α抗体（用于western blot）
英文名称：Anti-ER-Alpha
货号：bs-0254R
抗体来源：兔
克隆类型：多克隆
蛋白分子量：predicted molecular weight: 66kDa
纯化方法：affinity purified by Protein A
交叉反应：hu
测试应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
产品背景介绍：Estrogen and progesterone receptor are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements(EREs) alters transcription of ERE-containing genes. The carboxy terminal region of the estrgen receptor contains the ligand binding domain, the amino terminus serves as the transactivation domain, and the DNA binding domain is centrally located. Two forms of estrogen receptor have been identified, ER Alpha and ER Beta. ER Alpha and ER Beta have been shown to be differentially activated by various ligands. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-A and hPR-B), which arise from alternative splicing. In most cells, hPR-B functions as a transcriptional activator of progesterone-responsive gene, whereas hPR-A function as a transcriptional inhibitor of all steroid hormone receptors.类固醇受体（Steroid Receptors）产品图片Tissue/cell: Human laryngeal carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-ER-alpha Polyclonal Antibody, Unconjugated(bs-0254R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) stainingProtein: rat brain lysates, 30ug; Primary: Anti-ER-alpha (bs-0254R) at 1:100; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bse-0295G) at 1: 3000; DAB staining; Predicted band size : 66kDObserved band size : 66kDAntigen: bs-0254P, 0.2ug/100ul Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000; TMB(C-0024) staining; Read the data in MicroplateReader by 450nm.