APC anti-human CD7 Antibody
Catalog# / Size 343107 / 25 tests
343108 / 100 tests
Clone CD7-6B7
Workshop IV T-164
Other Names gp40
Isotype Mouse IgG2a, κ
Description CD7 is a 40 kD type I transmembrane glycoprotein also known as gp40. It is a member of the
immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic
progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia
(ALL) and some acute myeloid leukemia (AML) cells. CD7 crosslinking induces a calcium flux
in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase.
CD7 costimulation can induce cytokine secretion and modulate cellular adhesion.
Product Details
Reactivity Human
Antibody Type Monoclonal
Host Species Mouse
Immunogen KG1a cell line
Formulation Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% (w/v) BSA (origin
USA).
Preparation The antibody was purified by affinity chromatography, and conjugated with APC under optimal
conditions.
Concentration Lot-specific (please contact technical support for concentration and total µg amount, or use our Lookup
tool if you have a lot number.)
Storage & Handling The antibody solution should be stored undiluted between 2°C and 8°C, and protected from
prolonged exposure to light. Do not freeze.
Application FC - Quality tested
Recommended Usage Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric
analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100
µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser Red Laser (633 nm)
Application Notes Additional reported (for the relevant formats) applications include proteogenomics .
2
Application References
(PubMed link indicates
BioLegend citation)
1. Knapp W, et al. 1989. Leucocyte Typing IV:White Cell Differentiation Antigens. Oxford University
Press.
2. Peterson VM, et al. 2017. Nat. Biotechnol. 35:936. (PG)
Product Citations
1. Ishihara Y, et al. 2017. J Virol. 10.1128/JVI.00974-17. PubMed
2. Lv L, et al. 2019. Exp Ther Med. 17:1360. PubMed
3. Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
4. Minagawa A et al. 2018. Cell stem cell. 23(6):850-858 . PubMed
RRID AB_1877156 (BioLegend Cat. No. 343107)
AB_2291325 (BioLegend Cat. No. 343108)
Antigen Details
Structure Ig superfamily, type I transmembrane glycoprotein, 40 kD
Version: 1 Revision Date: 11/30/2012 
Distribution T cells, NK cells, thymocytes, hematopoietic progenitors, myeloid leukemic cell subsets
Function T cell activation
Ligand/Receptor Cytoplasmic domain associates with P13-kinase
Cell Type Hematopoietic stem and progenitors, Leukemia, NK cells, T cells, Thymocytes
Biology Area Immunology, Costimulatory Molecules
Molecular Family CD Molecules
Antigen References 1. Barclay N, et al. 1993. The Leucocyte Antigen FactsBook. Academic Press Inc. San Diego.
2. Stillwell R, et al. 2001. Immunol. Res. 24:31.
3. Rabinowich H, et al. 1994. J. Immunol. 152:517.
Gene ID 924
Related Protocols
Cell Surface Flow Cytometry Staining Protocol
Purified anti-human CD7, FITC anti-human CD7, PE anti-human CD7, PE/Cy5 anti-human CD7, PE/Cy7 anti-human CD7, Purified antihuman
CD7 (Maxpar® Ready), PerCP/Cyanine5.5 anti-human CD7, PE/Dazzle™ 594 anti-human CD7, APC/Fire™ 750 anti-human
CD7, APC anti-human CD7, Alexa Fluor® 647 anti-human CD7, TotalSeq™-A0066 anti-human CD7, Alexa Fluor® 700 anti-human CD7,
TotalSeq™-C0066 anti-human CD7
Other Formats
Product Data
Human peripheral blood lymphocytes
stained with CD7 (clone CD7-6B7) APC
(filled histogram) or mouse IgG2a, κ
isotype control APC (open histogram).
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