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Lifeline^® Normal Human Skin Fibroblasts – Neonatal (HDFn) provide an ideal cell system to establish serum free human feeder layers for human embryonic stem cell cultures or as a model to study wound healing, toxicology or basic cell biology.

Lifeline^® Dermal Fibroblasts – Neonatal are isolated from neonatal human foreskin and cryopreserved as primary cells to ensure the highest viability and plating efficiency.

• Our skin fibroblasts are quality tested in FibroLife® S2 or FibroLife Xeno-Free Medium to ensure proper growth and morphology over a period of at least 15 population doublings.
• Lifeline® dermal fibroblasts tested in both FibroLife^® Serum-Free Medium and FibroLife^® S2 Medium display optimal growth at rates equal to or greater than classical serum-supplemented medium.

500,000 cells per vial

Lifeline® dermal fibroblasts – neonatal are not exposed to antimicrobials or phenol red when cultured in the appropriate Lifeline FibroLife® medium, an advantage since these supplements can cause cell stress and “masking effects” that may negatively impact experimental results.

Dermal fibroblasts are quality tested for:

• Cell Viability: Minimum 70% viability when thawed from cryopreservation
• Sterility Testing: Negative for mycoplasma. Negative for bacterial and fungal growth.
• Virus Testing: Negative for HIV-1, HIV-2, HBV, and HCV by PCR