| Specificity |  | The gamma secretase is a multiprotein complex consisting of presenilin, nicastrin, Aph-1 and Pen-2; all four proteins are necessary for full proteolytic activity. The gamma secretase complex catalyzes cleavage of a number of integral membrane proteins including Amyloid precursor protein, Notch, Erb4 and E-cadherin. The results of several recent studies indicate that the functional gamma secretase activity mostly arises from a low abundance very high molecular weight complex (>670 kDa). These data suggest that additional proteins may be present in these functional complexes or the functional complex is a higher order multimer of presenilin, aph-1, pen-2 and nicastrin.
PRB-354P was raised against a peptide corresponding to the N-terminus of human PS1 and was subsequently antigen affinity purified. |
| References | | Gu Y, Sanjo N, Chen F, Hasegawa H, Petit A, Ruan X, Li W, Shier C, Kawarai T, Schmitt-Ulms G, Westaway D, St George-Hyslop P, Fraser PE. The presenilin proteins are components of multiple membrane-bound complexes which have different biological activities. J Biol Chem 279(30):31329-31336, 2004.
Yu G, Chen F, Nishimura M, Steiner H, Tandon A, Kawarai T, Arawaka S, Supala A, Song YQ, Rogaeva E, Holmes E, Zhang DM, Milman P, Fraser PE, Haass C, George-Hyslop PS. Mutation of conserved aspartates affects maturation of both aspartate mutant and endogenous presenilin1 and presenilin 2 complexes. J Biol Chem 275(35):27348-27353, 2000.
Chen F, Yang DS, Petanceska S, Yang A, Tandon A, Yu G, Rozmahel R, Ghiso J, Nishimura M, Zhang DM, Kawarai T, Levesque G, Mills J, Levesque L, Song YQ, Rogaeva E, Westaway D, Mount H, Gandy S, St George-Hyslop P, Fraser PE. Carboxyl-terminal fragments of alzheimer beta-amyloid precursor protein accumulate in restricted and unpredicted intracellular compartments in presenilin 1-deficient cells. J Biol Chem 275(47):36794-36802, 2000.
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