【Molecular Wt.】37 kDa

【Description】Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.

【Species Reactivity】Human;Mouse;Rat

【Tested applications】WB;ICC;IF;IHC;IP;FC

【Antibody type】Recombinant Rabbit monoclonal Antibody

【Immunogen】Recombinant protein

【Positive control】K562, Hela, LOVO, MCF-7, human tonsil tissue, human kidney tissue, human lung cancer tissue, mouse fallopian tube tissue, rat kidney tissue.

【Conjugate】Non-conjugated

【Alternative names】2PP2A antibody
HLA DR associated protein II antibody
HLA-DR-associated protein II antibody
I 2PP2A antibody
I-2PP2A antibody
I2PP2A antibody
IGAAD antibody
Inhibitor of granzyme A activated DNase antibody
Inhibitor of granzyme A-activated DNase antibody
Inhibitor of GzmA-activated DNase antibody
inhibitor-2 of protein phosphatase-2A antibody
IPP2A2 antibody
PHAPII antibody
Phosphatase 2A inhibitor I2PP2A antibody
protein phosphatase type 2A inhibitor antibody
Protein SET antibody
Set antibody
SET nuclear oncogene antibody
SET translocation antibody
SET translocation (myeloid leukemia-associated) antibody
SET_HUMAN antibody
TAF I antibody
TAF IBETA antibody
TAF-I antibody
TAFI antibody
Template activating factor I antibody
Template-activating factor I antibody
Template-Activating Factor-I, chromatin remodelling factor antibody

【Form】Liquid

【Stroage】Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

【Stroage Buffer】1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

【Isotype】IgG

【Purity】Protein A purified.

【Subcellular location】Nucleus. Cytoplasm. Endoplasmic reticulum.

【Background References】1. Beresford P J et al. Granzyme A activates an endoplasmic reticulum-associated caspase-independent nuclease to induce single-stranded DNA nicks. J Biol Chem 276:43285-43293 (2001).
2. Fan Z et al. Tumor suppressor NM23-H1 is a granzyme A-activated DNase during CTL-mediated apoptosis, and the nucleosome assembly protein SET is its inhibitor. Cell 112:659-672 (2003).

【DatasheetURL】http://www.huabio.com/upload/ET7107-18.pdf

【Uniprot ID】Q01105(Human) Q9EQU5(Mouse)

【Raised in】Rabbit

【Clone No.】JB60-34