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| 货号 | D6005 |
| 保存条件 | 常温 |
| 供应商 | 北京天漠科技开发有限公司 |
| 数量 | 大量 |
| 英文名 | ZR Fungal/Bacterial DNA Kit™ |
| 保质期 | ２年 |
| 规格 | 50preps |
简单、快速地从tough-to-lyse真菌和细菌中分离到humicfree,PCR-quality 的DNA整个过程仅10分钟。
The ZR Fungal/Bacterial DNA MiniPrep™ is designed for the simple and rapid isolation of DNA from tough-to-lyse fungi, including A. fumigatus, C. albicans, N. crassa, S. cerevisiae, S. pombe, as well as Gram (+/-) bacteria, algae, and protozoa. The procedure is easy and can be completed in minutes: fungal and/or bacterial samples are rapidly and efficiently lysed with our state of the art, ultra-high density BashingBeads™. Fast-Spin column technology is then used to isolate the DNA that is ideal for downstream molecular-based applications including PCR, array, etc.
| Format | Spin Column |
|---|---|
| DNA Recovery | Typically, up to 25 µg total DNA is eluted into ≥ 25 µl DNA Elution Buffer per sample. |
| Processing Time | 10 min |
| Equipment | Microcentrifuge, vortex, cell disruptor/pulverizer (optional). |
| DNA Size Limits | Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
| Sample Sources | 50-100 mg (wet weight) fungi or bacteria or up to 200 mg tissue. This equates to approximately 109bacterial cells, 108 yeast cells and 107 mammalian cells. Spores, pollen, nematodes, as well as other microorganisms can also be sampled. |
| DNA Purity | High quality DNA is eluted with DNA Elution Buffer making it perfect for PCR. A260/A280 >1.8 |
Fungal and bacterial DNA purification. DNA isolated from Saccharomyces cerevisiae (spores) and E. Coliusing the ZR Fungal/Bacterial DNA MiniPrep™ is high quality and structurally intact. Equivalent amounts of yeast and bacteria were processed using the ZR Fungal/Bacterial DNA MiniPrep™ or the kit from supplier A. Equal volumes of eluted DNA were then analyzed in a 0.8% (w/v) agarose/ethidium bromide ge |
