ATCC® Number: CCL-229™ 
Designations: LoVo 
Depositors: M Romsdahl 
Biosafety Level: 1 
Shipped: frozen 
Medium & Serum: See Propagation 
Growth Properties: adherent 
Organism: Homo sapiens (human) 
Morphology: epithelial

Source: Organ: colon 
Tumor Stage: Dukes' type C, grade IV 
Disease: colorectal adenocarcinoma 
Derived from metastatic site: left supraclavicular region 
Cellular Products: carcinoembryonic antigen (CEA) 908 ng/10 exp6 cells/10 days 
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Applications: transfection host (Roche FuGENE® Transfection Reagents) 
Tumorigenic: Yes 
Reverse Transcript: negative 
Oncogene: myc +; myb + ; ras +; fos +; p53 +; sis -; abl -; ros -; src - 
Antigenexpression: HLA A11, B15, B17, Cw1, Cw3; blood type B 
DNA Profile (STR): Amelogenin: XY 
CSF1PO: 10,11,13,14 
D13S317: 8, 11 
D16S539: 9, 12 
D5S818: 11, 12, 13 
D7S820: 9.3,10, 11 
TH01: 9.3 
TPOX: 8,9 
vWA: 17,18 
Cytogenetic Analysis: The stemline chromosome number is hyperdiploid with the 2S component occurring atabout2.7% and 3 marker chromosomes were common to all S metaphases. Karyotypes were generally homogeneous and stable. 
Isoenzymes: ES-D, 1 
G6PD, B 
PGD, A 
PGM1, 2 
PGM3, 1-2 
Age: 56 years 
Gender: male 
Comments: LoVo was initiated in 1971 from a fragment of a metastatic tumor nodule in the left supraclavicular region of a 56-year-old Caucasian male patient with a histologically proven diagnosis of adenocarcinoma of the colon. [1049] 
The cells are negative forexpressionof CSAp (CSAp-) and colon antigen 3. 
The line is positive forexpressionof c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. [22861] 
Myb, and fos oncogenes. [22861] 
N-myc and sis oncogeneexpressionwere not detected. [22861] 
Tumor specific nuclear matrix proteins CC-3 and CC-4 are expressed. [23341] 
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C 
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:10 is recommended 
Medium Renewal: 2 to 3 times per week 
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. 
Add fresh culture medium, aspirate and dispense into new culture flasks. 
Preservation: culture medium 95%; DMSO, 5% 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020 
References: 1047: Drewinko B, et al. Further biologic characteristics of a human carcinoembryonic antigen-producing colon carcinoma cell line. J. Natl. Cancer Inst. 61: 75-83, 1978. PubMed: 276641
1048: Drewinko B, Yand LY. Restriction of CEA synthesis to the stationary phase of growth of cultured human colon carcinoma cells. Exp. Cell Res. 101: 414-416, 1976. PubMed: 964319
1049: Drewinko B, et al. Establishment of a human carcinoembryonic antigen-producing colon adenocarcinoma cell line. Cancer Res. 36: 467-475, 1976. PubMed: 1260746
22861: Trainer DL, et al. Biological characterization and oncogeneexpressionin human colorectal carcinoma cell lines. Int. J. Cancer 41: 287-296, 1988. PubMed: 3338874
23341: Keesee SK, et al. Nuclear matrix proteins in human colon cancer. Proc. Natl. Acad. Sci. USA 91: 1913-1916, 1994. PubMed: 8127905
26057: Drewinko B, et al. Response of exponentially growing, stationary-phase, and synchronized cultured human colon carcinoma cells to treatment with nitrosourea derivatives. Cancer Res. 39: 2630-2636, 1979. PubMed: 445465
32913: Miranda L, et al. Isolation of the human PC6 gene encoding the putative host protease for HIV-1 gp160 processing in CD4+ T lymphocytes. Proc. Natl. Acad. Sci. USA 93: 7695-7700, 1996. PubMed: 8755538