【Molecular Wt.】21 kDa

【Description】The mammalian c-H-, c-K- and N-Ras proto-oncogenes encode guanine nucleotide-binding proteins that are ubiquitously expressed in vertebrate cells. c-H- and c-K-Ras are cellular homologs of the v-H and v-K-Ras sequences originally isolated from the Harvey and Kirsten strains of rat sarcoma virus. Ras-encoded proteins bind GDP and GTP with high affinity and possess a low level intrinsic GTPase activity that can be stimulated over 100-fold by interaction with cytosolic GTPase activating protein (GAP), a potential effector for Ras p21 function. Point mutations at amino acids 12, 13, 59 and 61 within domains responsible for GTP binding and hydrolysis activate Ras proteins to their oncogenic form and block the ability of the GTPase activity to be stimulated by GAP. Several additional proteins with GAP activity have been identified and shown to interact with p21 Ras or other members of the Ras gene family.

【Species Reactivity】Human;Mouse;Rat;zebrafish

【Tested applications】WB;ICC/IF;IP

【Antibody type】Recombinant Rabbit monoclonal Antibody

【Immunogen】recombinant protein

【Positive control】293T, MCF-7, Hela, PC-12, zebrafish.

【Conjugate】Non-conjugated

【Alternative names】C-BAS/HAS antibody
c-H-ras antibody
C-HA-RAS1 antibody
CTLO antibody
GTPase HRas antibody
GTPase KRas antibody
GTPase NRas antibody
H-Ras-1 antibody
H-RASIDX antibody
Ha-Ras antibody
HAMSV antibody
HRAS antibody
HRAS1 antibody
K RAS2A antibody
K RAS2B antibody
K RAS4A antibody
K RAS4B antibody
K-RAS antibody
KRAS antibody
KRAS1 antibody
KRAS2 antibody
N-RAS antibody
N-terminally processed antibody
NRAS antibody
NRAS1 antibody
p21ras antibody
RASH_HUMAN antibody
RASH1 antibody
RASK2 antibody
Transforming protein p21 antibody
v Ha ras Harvey rat sarcoma viral oncogene homolog antibody
v Ki ras2 Kirsten rat sarcoma viral oncogene homolog antibody
v ras neuroblastoma RAS viral oncogene homolog antibody

【Form】Liquid

【Stroage】Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

【Stroage Buffer】1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

【Isotype】IgG

【Purity】Protein A purified.

【Subcellular location】Cytoplasm, Cell membrane, Golgi apparatus, Nucleus.

【Background References】1. Zhou M et al. VPS35 binds farnesylated N-Ras in the cytosol to regulate N-Ras trafficking. J Cell Biol 214:445-58 (2016).
2. Meng X et al. RPL23 Links Oncogenic RAS Signaling to p53-Mediated Tumor Suppression. Cancer Res 76:5030-9 (2016).

【DatasheetURL】http://www.huabio.com/upload/ET1702-94.pdf

【Uniprot ID】P01111(Human) P01112(Human) P01116(Human) P08556(Mouse) P32883(Mouse) Q61411(Mouse) P08644(Rat) P20171(Rat) Q04970(Rat)

【Raised in】Rabbit

【Clone No.】JF10-11