| 货号 | 2108 |
| 保质期 | 详见说明书 |
| 数量 | 大量 |
| 应用范围 | W, IP, IF-IC, F |
| 适应物种 | H,M,R,Mk,C |
| 级别 | 详见MSDS文件 |
| 是否单克隆 | 单克隆 |
| 抗体英文名 | Src Antibody |
| 宿主 | Rabbit |
| 保存条件 | -20°c |
| 抗原 | synthetic peptide corresponding to residues close to the carboxy-terminus of human Src |
| 供应商 | CST |
| 规格 | 100 ul (10 western blots)/carrier free & custom formulation / quantity |
pathwaymore infoapplication referencesdatasheet PDFMSDS PDFprotocols
Applications Key: W=Western Blotting IP=Immunoprecipitation IF-IC=Immunofluorescence (Immunocytochemistry) F=Flow Cytometry
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey C=Chicken
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC F | H M R Mk (C) | Endogenous | 60 | Rabbit |
| Protocols | |
|---|---|
| Specificity / Sensitivity | Src Antibody detects endogenous levels of Src proteins. This antibody may cross-react with other Src family members, but does not cross-react with other non-family proteins. |
| Source / Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues close to the carboxy-terminus of human Src. Antibodies are purified by protein A and peptide affinity chromatography. Western BlottingWestern blot analysis of extracts from various cell lines using Src Antibody. Flow CytometryFlow cytometric analysis of Jurkat cells, using Src Antibody (blue) compared to a nonspecifc negative control antibody (red). IF-ICConfocal immunofluorescent analysis of MCF7 cells labeled with Src Antibody (red). Blue=DRAQ5® #4084 fluorescent DNA dye. |
| Background | The Src family of protein tyrosine kinases, which includes Src, Lyn, Fyn, Yes, Lck, Blk, and Hck, are important in the regulation of growth and differentiation of eukaryotic cells (1). Src activity is regulated by tyrosine phosphorylation at two sites, but with opposing effects. While phosphorylation at Tyr416 in the activation loop of the kinase domain upregulates enzyme activity, phosphorylation at Tyr527 in the carboxy-terminal tail by Csk renders the enzyme less active (2). |
| Application References |
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know! |
| Companion Products |
For Research Use Only. Not For Use In Diagnostic Procedures. |
