2822:

Immunoprecipitation, Western Blotting

PLCγ1 Antibody detects endogenous levels of total PLCγ1 protein. This antibody does not cross-react with PLCγ2.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human PLCγ1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-stimulated for the indicated times, using PLCγ1 Antibody.

IP

Immunoprecipitation of PLCγ1 from untreated or PDGF treated NIH/3T3 cells followed by Western blot analysis, using PLCγ1 Antibody.

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ and PLCε. The PLCβ subfamily includes four members, PLCβ1-4. All four members of the subfamily are activated by α- or β-γ-subunits of the heterotrimeric G-proteins (2,3).Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of Ser1105 by PKA or PKC inhibits PLCβ3 activity (4,5). Ser537 of PLCβ3 is phosphorylated by CaMKII, and this phosphorylation may contribute to the basal activity of PLCβ3. PLCγ is activated by both receptor and nonreceptor tyrosine kinases (6).PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783 and 1245 (7). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (8).

1. Singer, W. D. et al. (1997) Annu. Rev. Biochem. 66, 475-509.
2. Smrcka, A. V. et al. (1991) Science 251, 804-807.
3. Taylor, S. J. et al. (1991) Nature 350, 516-518.
4. Yue, C. et al. (1998) J. Biol. Chem. 273, 18023-18027.
5. Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.
6. Margolis, B. et al. (1989) Cell 57, 1101-1107.
7. Kim, H. K. et al. (1991) Cell 65, 435-441.
8. Wang, Z. et al. (1998) Mol. Cell. Biol. 18, 590-597.

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• 2821 Phospho-PLCγ1 (Tyr783) Antibody
• 3872 PLCγ2 Antibody
• 2481 Phospho-PLCbeta3 (Ser537) Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody

For Research Use Only. Not For Use In Diagnostic Procedures.