3545:

Western Blotting

Nod1 Antibody detects endogenous levels of Nod1 protein.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of Nod1 protein. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from SR (human), 4T1 (mouse) and C6 (rat) cell lines using Nod1 Antibody.

Nod1/CARD4 is a cytosolic protein structually related to Apaf-1 and plant drug-resistance proteins that has been implicated in apoptosis and inflammatory responses to certain pathogenic bacteria (1-3). It contains an amino-terminal caspase recruitment domain (CARD) that is linked to a central nucleotide-binding domain (NBD; also known as a NOD domain) and is followed by carboxy-terminal leucine-rich repeats (LRR) (1). Like Apaf-1, Nod1 induces apoptosis by a CARD/NBD-dependent activation of caspase-9 (1). The primary function of Nod1 is thought to be as a sensor for certain pathogenic microbes and triggering inflammatory responses including the activation of NF-κB and JNK pathways (4-6). The LRR of Nod1 appears to be involved in recognition of microbial components and the CARD domain induces NF-κB activation in cooperation with the CARD containing kinase, RICK/RIP2/CARDIAK (1,5,6). Mutations in Nod1 have been linked increased susceptibility to asthma (7) and inflammatory bowel disease (8).

1. Inohara, N. et al. (1999) J. Biol. Chem. 274, 14560-14567.
2. Inohara, N. and Nuñez, G. (2003) Nat. Rev. Immunol. 3, 371-382.
3. Fritz, J.H. et al. (2006) Nat .Immunol. 7, 1250-1257.
4. Girardin, S.E. et al. (2001) EMBO Rep. 2, 736-742.
5. Inohara, N. et al. (2001) J. Biol. Chem. 276, 2551-2554.
6. Inohara, N. et al. (2000) J. Biol. Chem. 275, 27823-27831.
7. Hysi, P. et al. (2005) Hum. Mol. Genet. 14, 935-941.
8. McGovern, D.P. et al. (2005) Hum. Mol. Genet. 14, 1245-1250.

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