3852:

IHC / Paraffin, Western Blotting

MMP-9 Antibody detects full length (proenzyme, 92 kDa) and cleaved (active enzyme, 84 kDa) MMP-9.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys115 of human MMP-9. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of partially purified, concentrated, serum-free medium containing human MMP-9, using MMP-9 Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human benign meningioma, using MMP-9 Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human squamous cell carcinoma of the esophagus, using MMP-9 Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma, using MMP-9 Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using MMP-9 Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

The matrix metalloproteinases (MMPs) are a family of proteases that target many extracellular proteins including other proteases, growth factors, cell surface receptors and adhesion molecules (1). Among the family members, MMP-2, MMP-3, MMP-7 and MMP-9 have been characterized as important factors for normal tissue remodeling during embryonic development, wound healing, tumor invasion, angiogenesis, carcinogenesis and apoptosis (2-4). MMP activity correlates with cancer development (2). One mechanism of MMP regulation is transcriptional (5). Once synthesized, MMP exists as a latent proenzyme. Maximum MMP activity requires proteolytic cleavage to generate active MMPs by releasing the inhibitory propeptide domain from the full length protein (5).

1. McCawley, L.J. and Matrisian, L.M. (2001) Curr. Opin. Cell Biol. 13, 534-540.
2. Coussens, L.M. et al. (2002) Science 295, 2387-2391.
3. Sternlicht, M.D. et al. (1999) Cell 98, 137-146.
4. Vu, T.H. et al. (1998) Cell 93, 411-422.
5. Nagase, H. et al. (1990) Biochemistry 29, 5783-5789.

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

• 2270 MMP-9 (G657) Antibody
• 4022 MMP-2 Antibody
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 8112 SignalStain^® Antibody Diluent

For Research Use Only. Not For Use In Diagnostic Procedures.