4727:

Flow, IHC / Paraffin, Immunofluorescence, Immunoprecipitation, Western Blotting

c-Rel Antibody detects endogenous levels of total c-Rel protein. Cross reactivity was not detected with other family members.

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human c-Rel. Antibodies were purified by protein A and peptide affinity chromatography.

Western Blotting

Western blot analysis of extracts from K562, Raji and 293 cells, using c-Rel Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using c-Rel Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded Hodgkin's lymphoma, showing nuclear and cytoplasmic localization using c-Rel Antibody.

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human skin, using c-Rel Antibody.

Flow Cytometry

Flow cytometric analysis of K562 cells using c-Rel Antibody (blue) compared to a nonspecific negative control antibody (red).

IF-IC

Confocal immunofluorescent analysis of HeLa cells, untreated (left) or TNFα-treated (#2169, 20 ng/ml for 20 min, right), using c-Rel Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red).

Transcription factors of the nuclear factor κ B (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which is then translocated to the nucleus (9-11).

1. Baeuerle, P.A. and Henkel, T. (1994) Annu Rev Immunol 12, 141-79.
2. Baeuerle, P.A. and Baltimore, D. (1996) Cell 87, 13-20.
3. Haskill, S. et al. (1991) Cell 65, 1281-9.
4. Thompson, J.E. et al. (1995) Cell 80, 573-82.
5. Whiteside, S.T. et al. (1997) EMBO J 16, 1413-26.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Scherer, D.C. et al. (1995) Proc Natl Acad Sci USA 92, 11259-63.
8. Chen, Z.J. et al. (1996) Cell 84, 853-62.
9. Senftleben, U. et al. (2001) Science 293, 1495-9.
10. Coope, H.J. et al. (2002) EMBO J 21, 5375-85.
11. Xiao, G. et al. (2001) Mol Cell 7, 401-9.

• Hasler, U. et al. (2008) J Biol Chem 283, 28095-105. Applications: Western Blotting
• Kwong, C. et al. (2011) J Immunol 186, 1781-9. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

• 4764 NF-κB p65 (C22B4) Rabbit mAb
• 4922 RelB (C1E4) Rabbit mAb
• 4717 NF-κB1 p105 Antibody
• 3035 NF-κB1 p105/p50 Antibody
• 4882 NF-κB2 p100/p52 Antibody
• 3017 NF-κB2 p100/p52 (18D10) Rabbit mAb (Human Specific)
• 3033 Phospho-NF-κB p65 (Ser536) (93H1) Rabbit mAb
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9243 NF-κB Control Cell Extracts
• 4766 NF-κB Family Member Antibody Sampler Kit
• 9936 NF-κB Pathway Sampler Kit

For Research Use Only. Not For Use In Diagnostic Procedures.