木瓜酶

Papain 10108014001

from Carica papaya

产品描述

Working concentration: 0.05 to 0.5 mg/ml

Specificity

Papain is a cysteine endopeptidase, which hydrolyzes proteins, peptides, amides, and esters of amino acids and peptides, specifically at bonds involving the amino acids Arg, Lys, Glu, His, Gly, and Tyr. Upon prolonged incubation, further bonds are split. It also exhibits esterase and transamidase activity and is used for peptide synthesis.

Physical form

Suspension, crystalline, nonsterile

Application

Use Papain for the complete proteolytic cleavage of proteins, limited hydrolysis of native immunoglobulins, and tissue dissociation (together with collagenase, esterase, and trypsin). The enzyme solubilizes integral membrane proteins and produces glycopeptides from purified proteoglycans. The addition of cysteine (approximately 0.5% [w/v]) is essential for enzyme activity.