CATALOGUE NUMBER

PRO-2479

SYNONYMS

Anti-Muellerian hormone, AMH, Muellerianinhibiting substance, MIS, MIF.

INTRODUCTION

Anti-Mullerian Hormone also known as AMH is a member of the TGF-beta family. AMH is a glycoprotein which is produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. AMH inhibits the growth of tumors derived from tissues of Muellerian duct origin. Moreover, AMH participates in Leydig cell differentiation and function in addition to follicular development in adult females.

DESCRIPTION

AMH Human Recombinant produced in E. coli is a single, non-glycosylated polypeptide chain (a.a 452-560) containing 118 amino acids including a 9 a.a N-terminal His tag. The total molecular mass is 12.8kDa (calculated).

SOURCE

E. coli

PHYSICAL APPEARANCE

FORMULATION

AMH filtered (0.4 µm) and lyophilized in 1mM HCl and 5% (w/v) trehalose.

SOLUBILITY

It is recommended to add 200µl deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. AMH is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

STABILITY

Store lyophilized protein at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after one week at 4°C.

PURITY

Greater than 95.0% as determined by SDS-PAGE.

AMINO ACID SEQUENCE

MKHHHHHHAS AGATAADGPC ALRELSVDLR AERSVLIPET YQANNCQGVC GWPQSDRNPR YGNHVVLLLK MQARGAALAR PPCCVPTAYA GKLLISLSEE RISAHHVPNM VATECGCR

USAGE

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.