G蛋白结合蛋白3抗体价格

¥1000 - 3500
上海博湖
进口、国产
2021-09-10 05:59

上海博湖生物科技有限公司

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上海博湖生物科技有限公司
韩丽君
18117197628 021-57763112
3004987436@qq.com
产品属性
抗体名G蛋白结合蛋白3抗体价格
抗体英文名Anti-SPG3A/Atlastin
靶点详见说明书
浓度1mg/1ml
应用范围WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
宿主详见说明书
供应商上海博湖
数量大量
级别详见说明书
目录编号详见说明书
抗原来源Rabbit
保质期详见说明书
适应物种详见说明书
标记物详见说明书
克隆性多克隆
保存条件Store at -20 °C
形态详见说明书
亚型IgG
免疫原KLH conjugated synthetic peptide derived from human SPG3A/Atlastin (201-300aa)
规格0.2ml/200μg
产品说明
英文名称 Anti-SPG3A/Atlastin
中文名称G蛋白结合蛋白3抗体价格
AD FSP; atl1; ATLA1_HUMAN; Atlastin GTPase 1; Atlastin-1; Atlastin1; Brain specific GTP binding protein; Brain-specific GTP-binding protein; FSP1; GBP-3; GBP3; GTP-binding protein 3; Guanine nucleotide-binding protein 3; Guanylate binding protein 3; hGBP3; HSN1D; Spastic paraplegia 3 protein A; SPG 3A; SPG3; SPG3A. 


G蛋白结合蛋白3抗体价格 1mg/1ml
0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Rabbit
产品类型一抗
研究领域细胞生物神经生物学结合蛋白 G蛋白偶联受体 G蛋白信号
蛋白分子量 predicted molecular weight: 64kDa
Lyophilized or Liquid
KLH conjugated synthetic peptide derived from human SPG3A/Atlastin (201-300aa)
IgG
纯化方法 affinity purified by Protein A
Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4


G蛋白结合蛋白3抗体价格产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
(石蜡切片需做抗原修复)
 not yet tested in other applications.
 optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. 
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 
产品介绍 Atlastins are Golgi-localized, integral membrane proteins that function as GTPases. The Atlastin proteins, also designated SPG3A and guanylate-binding protein 3, comprise a Dynamin superfamily that plays a role in axonal maintenance. Hereditary spastic paraplegia (HSP) is an inherited neurodegenerative disorder that is characterized by retrograde axonal degeneration. HSP primarily affects long corticospinal neurons and causes spastic lower extremity weakness. Spastin, a microtubule (MT)-severing AAA ATPase, is a binding partner of Atlastin that is involved in membrane dynamics. This Spastin/Atlastin binding may be involved in the biochemical pathway that leads to HSP development. Mutations in the Atlastin gene (SPG3A) account for approximately 10% of all autosomal dominant HSPs, while mutations in the Spastin gene (SPG4) account for almost 40%.

Function : GTPase tethering membranes through formation of trans-homooligomer and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis. May also regulate Golgi biogenesis. May regulate axonal development.
Subunit : Homooligomer. Interacts (via N-terminal region) withMAP4K4 (via CNH regulatory domain). Interacts with REEP5, RTN3 andRTN4 (via the transmembrane region). Interacts with SPAST;interaction is direct. May interact with TMED2. Interacts withREEP1.
Subcellular Location : Endoplasmic reticulum membrane. Golgi apparatus membrane. Cell projection > axon.
Tissue Specificity : Expressed predominantly in the adult and fetal central nervous system. Measurable expression in all tissues examined, although expression in adult brain is at least 50-fold higher than in other tissues. Detected predominantly in pyramidal neurons in the cerebral cortex and the hippocampus of the brain. Expressed in upper and lower motor neurons (at protein level).
DISEASE : Defects in ATL1 are the cause of spastic paraplegia autosomal dominant type 3 (SPG3) [MIM:182600]; also known as Strumpell-Lorrain syndrome. Spastic paraplegia is a degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs.
Similarity : Belongs to the GBP family. Atlastin subfamily.
Database links : UniProtKB/Swiss-Prot: Q8WXF7.1


G蛋白结合蛋白3抗体价格抗体的鉴定:
1)抗体的效价鉴定:不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2)抗体的特异性鉴定:抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
如果所用抗原浓度IC50浓度为pg/,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3)亲和力:是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属:
兔抗单克隆抗体,鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml抗体及相关标记抗体:HRP标记抗体,Biotin标记抗体,Gold标记抗体,RBITC标记抗体,AP标记抗体,FITC标记抗体,Cy3标记抗体,Cy5标记抗体,Cy5.5标记抗体,Cy7标记抗体,PE标记抗体,PE-Cy3标记抗体,PE-Cy5标记抗体,PE-Cy5.5标记抗体,PE-Cy7标记抗体,APC标记抗体,AlexaFluor 350标记抗体,Alexa Fluor 488标记抗体,Alexa Fluor 555标记抗体,Alexa Fluor647标记抗体抗体的交叉反应:人,小鼠,大鼠,鸡,狗,猪,羊,牛,兔等...... 抗体的应用:可以用于做石蜡切片免疫组化,冰冻切片免疫组化,ElisaWB,免疫荧光等实验。
G蛋白结合蛋白3抗体价格实验原理 :
1)特异性结合抗原:抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞,通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而,抗体可通过与病毒或毒素的特异性结合,直接发挥中和病毒的作用。
2)活补体:IgMIgG1IgG2IgG3可通过经典途径激活补体,凝聚的IgAIgG4IgE可通过替代途径激活补体。
3)结合细胞:不同类别的免疫球蛋白,可结合不同种的细胞,参与免疫应答。
4)可通过胎盘及粘膜:免疫球蛋白GIgG)能通过胎盘进入胎儿血流中,使胎儿形成自然被动
免疫。免疫球蛋白AIgA)可通过消化道及呼吸道粘膜,是粘膜局部抗感染免疫的主要因素。
5)具有抗原性:抗体分子是一种蛋白质,也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子,各具有不同的抗原性。
6)抗体对理化因子的抵抗力与一般球蛋白相同:不耐热,6070即被破坏。各种酶及能使蛋白质凝固变性的物质,均能破坏抗体的作用。抗体可被中性盐类沉淀。在生产上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白,再经透析法将其纯化。


Anti-SP /FITC 荧光素标记P物质抗体IgGMulti-class antibodies规格: 0.2ml
Cytomegalovirus (CMV)PP65 细胞巨化病毒(多肽)Multi-class antibodies规格: 0.5mg
神经再生相关蛋白抗体 Anti-P311 protein 0.1ml
phospho-14-3-3 protein zeta + delta (Ser58) 英文名称:14-3-3 α/β/ζ抗体 0.1ml
phospho-E2F1 (Ser337) 英文名称:化转录因子E2F-1抗体 0.1ml
Rhesus antibody Rh phospho-NFKBIA(Tyr305) IKB alpha抗体规格 0.1ml
Cytomegalovirus (CMV)PP65 细胞巨化病毒(多肽)Multi-class antibodies规格: 0.5mg
UPAR 人尿激酶样纤溶酶原活化物受体Multi-class antibodies规格: 48T

Anti-Cyclooxygenase 2/COX2 前列腺素过氧化物合成酶2抗体Multi-class antibodies规格: 0.1ml
Rhesus antibody Rh NMT1 豆蔻酰基转移酶1抗体规格 0.2ml
Human cartilage oligomeric protein,COMP ELISA Kit 人软骨寡聚蛋白 96T
THBS1 英文名称:血小板反应蛋白抗体 0.1ml
CSPS 英文名称:磺基转移酶CSPS抗体 0.2ml
Anti-Cyclooxygenase 2/COX2 前列腺素过氧化物合成酶2抗体Multi-class antibodies规格: 0.1ml
Dog IgM/Cy3 Cy3标记的兔抗犬IgM抗体 0.1ml

FAM59B 英文名称: FAM59B蛋白抗体 0.2ml
三鸟苷环水解酶抗体 Anti-GTP-CH-1 0.2ml
Anti-SIAH1/FITC 荧光素标记泛素连接酶Siah1抗体IgGMulti-class antibodies规格: 0.2ml
Rhesus antibody Rh phospho-RUNX2(Ser451) 化成骨特异性转录因子抗体规格 0.1ml
Rabbit Anti-rat IgM 兔抗大鼠IgM (亲和层析纯化)Multi-class antibodies规格: 1mg
SMMC-7721(人肝癌细胞) 5×106cells/瓶×2

正常大鼠肾细胞;NRK
EFNB2A Others Danio rerio (zebrafish) 斑马鱼 EFNB2A / Ephrin B2a 人细胞裂解液 (阳性对照)
WI-38细胞,人胚肺细胞小鼠肺成纤维细胞,L929细胞 CL-00025637(人膀胱癌细胞)5×106cells/瓶×2
雪旺氏细胞Many types of cells包装:5 × 105次方(1ml)
HDMEC-c 人真皮微血管内皮细胞(HDMEC)来自少年者 500,000cells 肺微血管内皮细胞Many types of cells包装:5 × 105次方(1ml)
绵羊皮肤细胞;OAR-S1

IL13RA2 Others Canine IL13RA2 / IL13R 人细胞裂解液 (阳性对照)
CL-0141LLC-MK2(恒河猴肾细胞)5×106cells/瓶×2
B16-F1细胞,鼠黑色素瘤细胞系血细胞瘤细胞系,Ramos细胞仓鼠/小鼠杂交瘤细胞;145-2C11
AR42J(大鼠胰腺外分泌细胞) 5×106cells/瓶×2
CXADR Others Human CXADR / CAR 人细胞裂解液 (阳性对照)
G蛋白结合蛋白3抗体价格SMMC-7721(人肝癌细胞) 5×106cells/瓶×2

正常大鼠肾细胞;NRK
EFNB2A Others Danio rerio (zebrafish) 斑马鱼 EFNB2A / Ephrin B2a 人细胞裂解液 (阳性对照)
WI-38细胞,人胚肺细胞小鼠肺成纤维细胞,L929细胞 CL-00025637(人膀胱癌细胞)5×106cells/瓶×2
雪旺氏细胞Many types of cells包装:5 × 105次方(1ml)
HDMEC-c 人真皮微血管内皮细胞(HDMEC)来自少年者 500,000cells 肺微血管内皮细胞Many types of cells包装:5 × 105次方(1ml)