Recombinant Protein A, His-tag

¥1499
联迈生物
中国/上海
2021-09-10 09:44

上海联迈生物工程有限公司

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上海联迈生物工程有限公司
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产品属性
数量大量
英文名Recombinant Protein A, His-tag
保质期一年
供应商上海联迈生物工程有限公司
保存条件-20℃或更低温度保存
规格100mg
产品说明
Recombinant Protein A, His-tag
产品编号产品名称产品包装产品价格
LM5003-100mgRecombinant Protein A, His-tag100mg1499.00
SpeciesGene IDAccessionSourceLengthMWTag
E. coli306aa34.7kDaHis
About this Protein
NameRecombinant Protein A, His-tag (Recombinant Protein A, His-tag; Protein A, His-tag);重组蛋白A,His标签
SynonymsN/A
Purity>97% by SDS-PAGE and HPLC analyses
Biological ActivityThe recombinant Protein A is a genetically engineered protein containing 5 IgG-binding regions of protein A. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A to ensure the maximum specific IgG binding. The recombinant Protein A is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.
Physical AppearanceSterile Filtered White lyophilized (freeze-dried) powder.
FormulationLyophilized from additive free solution.
EndotoxinLess than 0.1EU/μg of Protein A, His-tag as determined by LAL method.
ReconstitutionDissolve in distilled water or saline.
CategoryProtein A
BackgroundProtein A is a cell wall component produced by several strains of Staphylococcus aureus that consists of a single polypeptide chain and contains little or no carbohydrate. Recombinant Protein A is produced in E. Coli and functions essentially the same as native Protein A. It consists of 5 IgG-binding domains E-D-A-B-C aligned in series.
Amino Acid SequenceMNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE HHHHHH
包装清单:
产品编号产品名称包装
LM5003-10mgRecombinant Protein A, His-tag10mg
LM5003-100mgRecombinant Protein A, His-tag100mg
LM5003-1gRecombinant Protein A, His-tag1g
-说明书1
保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以首次配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
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