Her2 / ErbB2 antibody [C2C3], C-term detects Her2 / ErbB2 protein at cell membrane in human breast carcinoma by immunohistochemical analysis.
Sample: Strong positive (++), low positive (+) and negative tissue slides cores assessed using Quantitative Digital Pathology.
Her2 / ErbB2 antibody [C2C3], C-term (GTX100509) diluted at 1:500, and competitor's antibody (CST#4290) diluted at 1:100.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Wild-type (WT) and Her2 / ErbB2 knockout (KO) HeLa cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 antibody (GTX100509) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Non-transfected (–) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 antibody (GTX100509) diluted at 1:7000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 antibody [C2C3], C-term (GTX100509) diluted at 1:10000.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 antibody [C2C3], C-term (GTX100509) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Her2 / ErbB2 antibody [C2C3], C-term (GTX100509) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.