Protein Name
Cytotoxic T-lymphocyte protein 4 (Cytotoxic T-lymphocyte-associated antigen 4) (CTLA-4) (CD antigen CD152)
Synonyms
Cytotoxic T-Lymphocyte-Associated Protein 4, Insulin-Dependent Diabetes Mellitus 12, Celiac Disease 3, CELIAC3, IDDM12, CTLA-4, CD152, GRD4, Ligand And Transmembrane Spliced Cytotoxic T Lymphocyte Associated Antigen 4, Cytotoxic T Lymphocyte Associated Antigen 4 Short Spliced Form
Species
Human
Source
Expression System
Escherichia coli (E.coli)
Expressed Region
Lys36-Asp161
Conjugation/Tag
N-terminal histidine tag
Preparation
Purification
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Purity determined by
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Specifications
Formulation
Supplied as a 0.45 µm filtered solution in 50 mM Na2HPO4 (pH 7.4), 0.5 M NaCl, 440 mM imidazole, 6 M urea.
Concentration
Lot specific (see the label on the vial), determined by Bio-Rad protein assay dye reagent concentrate.
Endotoxin Level
Not determined
Recommended Applications
Functional Assay, Protein-protein Interaction, Post-translational Modifications, ELISA, EIA, Western Blotting, Dot Blotting, Immunoprecipitation, Protein Array, etc.
Stability & Storage
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
