【Molecular Wt.】100 KDa

【Description】Guanine nucleotide exchange factor (GEF) that catalyzes the exchange of GDP for GTP. Promotes guanine nucleotide exchange on the Rho family members of small GTPases, like RHOA, RHOC, RAC1 and CDC42. Required for signal transduction pathways involved in the regulation of cytokinesis. Component of the centralspindlin complex that serves as a microtubule-dependent and Rho-mediated signaling required for the myosin contractile ring formation during the cell cycle cytokinesis. Regulates the translocation of RHOA from the central spindle to the equatorial region. Plays a role in the control of mitotic spindle assembly; regulates the activation of CDC42 in metaphase for the process of spindle fibers attachment to kinetochores before chromosome congression. Involved in the regulation of epithelial cell polarity; participates in the formation of epithelial tight junctions in a polarity complex PARD3-PARD6-protein kinase PRKCQ-dependent manner. Plays a role in the regulation of neurite outgrowth. Inhibits Phenobarbital (PB)-induced NR1I3 nuclear translocation. Stimulates the activity of RAC1 through its association with the oncogenic PARD6A-PRKCI complex in cancer cells, thereby acting to coordinately drive tumor cell proliferation and invasion. Also stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.

【Species Reactivity】Human;Mouse;Rat;Chicken;Dog;Cow;Rabbit

【Tested applications】WB

【Antibody type】Rabbit polyclonal Antibody

【Immunogen】KLH conjugated synthetic peptide derived from human ECT2 781-883/914

【Positive control】Expressed in lung epithelial cells (at protein level). Expressed in squamous cell carcinoma, primary non-small cell lung cancer tumors and lung adenocarcinoma. [INDUCTION] Up-regulated by calcium in cells forming cell-cell contact sites. Up-regulated by DNA damaging agents like H(2)O(2) or ionizing radiation (IR).

【Conjugate】Non-conjugated

【Alternative names】ECT 2
ECT2
ECT-2
ECT2 protein
Epithelial cell transforming sequence 2
Epithelial cell transforming sequence 2 oncogene
Epithelial cell transforming sequence 2 oncogene protein
FLJ10461
MGC138291
Protein ECT2
ECT2_HUMAN
Epithelial cell-transforming sequence 2 oncogene.

【Form】Liquid

【Stroage】Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

【Stroage Buffer】0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

【Isotype】IgG

【Purity】affinity purified by Protein A

【Subcellular location】Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Cleavage furrow. Midbody. Cell junction. Cell junction, tight junction. Note=Sequestered within the nucleus during interphase. Dispersed throughout the cytoplasm upon breakdown of the nuclear envelope during mitosis. Colocalizes with the centralspindlin complex to the mitotic spindles during anaphase/metaphase, the cleavage furrow during telophase and at the midbody at the end of cytokinesis. Colocalized with RhoA at the midbody. Its subcellular localization to tight junction is increased by calcium. Localized predominantly in the cytoplasm of numerous carcinoma cells.

【Background References】

【DatasheetURL】http://www.huabio.com/upload/ER1907-92.pdf

【Uniprot ID】SwissProt: Q9H8V3 Human

【Raised in】Rabbit

【Clone No.】