Rat HB- EGF ELISA Kit

Product Name:Rat HB- EGF ELISA Kit
Packing:96T

Catalog No.:ELA-E1479r

Gene Name:HB-EGF

Detect Range:0.156-10 ng/mL

Sensitivity:0.054 ng/mL

Target Protein Name:HB-EGF

Alternative Name:HB-EGF
Sample type:serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA type:Sandwich ELISA Kit
Product Description:Rat HB- EGF ELISA Kit allows for the in vitro quantitative determination of HB-EGF concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

ELISA Test Principle:
The microtiter plate provided in Human LRP2 ELISA Kit has been pre-coated with an LRP2 antibody specific to Human Low-density lipoprotein receptor-related protein 2 .Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for Human Low-density lipoprotein receptor-related protein 2 and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human Low-density lipoprotein receptor-related protein 2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Human Low-density lipoprotein receptor-related protein 2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NOTE:FOR RESEARCH USE ONLY; NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS! PLEASE READ ENTIRE PROCEDURE!

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