| Designations: | PT67 [10A1-based retrovirus packaging cell line] |
| Depositors: | Fred Hutchinson Cancer Res. Cntr. |
| Biosafety Level: | 2 [cells contain SV-40 viral DNA sequences ] |
| Shipped: | frozen |
| Medium & Serum: | See Propagation |
| Growth Properties: | adherent |
| Organism: | Mus musculus (mouse) |
| Morphology: | |
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.CRL-12284 PT67 逆转录酶病毒包装细胞 |
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| Applications: | transfection host (Roche FuGENE® Transfection Reagents) |
| Comments: | PT67 is a retrovirus packaging cell line derived from TK- NIH/3T3 cells.
The cells produce replication-defective retroviral vector particles capable of binding to the gibbon ape leukemia virus receptor Glvr-1 (Pit-1) or the amphotropic retrovirus receptor Ram-1 (Pit-2) on target cells. [32364] |
| Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks. |
| Preservation: | culture medium 95%; DMSO, 5% |
| Related Products: | Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020 |
| References: | 32364: Miller AD, Chen F. Retrovirus packaging cells based on 10AI murine leukemia virus for production of vectors that use multiple receptors for cell entry. J. Virol. 70: 5564-5571, 1996. PubMed: 8764070
46921: Miller AD. 10A1 Retroviral packaging cells and uses thereof. US Patent 5,766,945 dated Jun 16 1998 |
