| Designations: | FDC-P1 |
| Depositors: | University of Virginia Patent Foundation |
| Biosafety Level: | 1 |
| Shipped: | frozen |
| Medium & Serum: | See Propagation |
| Growth Properties: | suspension |
| Organism: | Mus musculus (mouse) |
| Morphology: | lymphoblast
|
| Source: | Organ: bone marrow
Strain: DBA/2
Disease: normal |
| Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
|
| Applications: | transfection host (Nucleofection technology from Lonza) |
| Antigen Expression: | Thy-1.2 (24%) |
| Cytogenetic Analysis: | diploid |
| Comments: | The FDC-P1 cell line was established from long term culture of normal DBA/2 bone marrow cells in medium conditioned by WEHI-3 cells (see ATCC TIB-68).
They are dependent upton WEHI-3 conditioned medium (WEHI-3CM) for continued growth. |
| Propagation: | ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 65%; fetal bovine serum, 10% supplemented with 25% mouse Interleukin-3 culture supplement. (Supplement purchased from BD Biosciences, Catalog No. 354040).
Temperature: 37.0°C |
| Subculturing: | Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Medium Renewal: Every 2 to 3 days |
| Preservation: | Freeze medium: Complete growth medium, 90%; DMSO, 10%
Storage temperature: liquid nitrogen vapor temperature |
