| Designations: | SCaBER | | Depositors: | C O'Toole | | Biosafety Level: | 1 | | Shipped: | frozen | | Medium & Serum: | See Propagation | | Growth Properties: | adherent | | Organism: | Homo sapiens (human) | | Morphology: | epithelial
| | Source: | Organ: urinary bladder
Disease: squamous cell carcinoma | | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | | Tumorigenic: | Yes | | DNA Profile (STR): | Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 11,12
D16S539: 11,12
D5S818: 12,13
D7S820: 8,10
THO1: 7,9
TPOX: 9,11
vWA: 16 | | Cytogenetic Analysis: | 2n = 46. The stemline chromosome number is hypotriploid with the 2S component occurring at 9.2%., Eleven markers [t(1;?) del (1)(p13), t(3;?), i(4q), t(8;?), t(9;?), M6, t(1;11), t(21;22), M13, and t(20;?)] and 1 to 3 small chromosome fragments were common to most S metaphases., At least one Y chromosome was always detectable. Number 3 was nullisomic. Neither homogenously staining regions (HSR) nor double minutes (DM) were seen. | | Isoenzymes: | AK-1, 1
ES-D, 1
G6PD, A
GLO-I, 1-2
Me-2, 2
PGM1, 1-2
PGM3, 1-2 | | Age: | 58 years | | Gender: | male | | Ethnicity: | Black | | Comments: | Contains the unusual type A isoenzyme of glucose-6-phosphate dehydrogenase. | | Propagation: | ATCC complete growth medium: Minimum essential medium (Eagle) with 2 mM L-glutamine and Earle's BSS adjusted to contain 1.5 g/L sodium bicarbonate, 0.1 mM non-essential amino acids, and 1.0 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C | | Subculturing: | Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution, remove trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes.
Add fresh medium, aspirate and dispense into new flasks.
Subculture every 6 to 8 days. | | Preservation: | Culture medium, 95%; DMSO, 5% | | References: | 21849: O'Toole CHuman bladder cancer lines: HLA Class I and Class II antigen expression and susceptibility to cytostatic and cytotoxic effects in vitroIn: O'Toole CIn vitro models for cancer researchvol. IVBoca Raton, FLCRC Presspp. 103-125.
22852: O'Toole C, et al. A cell line (SCABER) derived from squamous cell carcinoma of the human urinary bladder. Int. J. Cancer 17: 707-714, 1976. PubMed: 947851
24381: Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047 |
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