| 数量 | 3-4周 |
| 供应商 | 博士德生物 |
| 检测方法 | 酶联免疫竞争法 |
| 应用 | Serum |
| 适应物种 | Human |
| 规格 | 96T |
This Bosterbio ELISA kit is designed to measure the concentration of Estrodial (E2) in human serum by Competitive ELISA (Enzyme-Linked Immunosorbent Assay). A goat anti-rabbit coated microplate is used to prepare a solid phase secondary antibody, and then add samples, HRP Conjugated Human Estrodial (E2) and Rabbit anti- Human Estrodial (E2) Antibody to form a coated secondary antibody-anti- Estrodial (E2) antibody- Estrodial (E2) (HRP) complex. The amount of bound peroxidase conjugate is inversely proportional to the concentration of estradiol in the sample. After color development, the absorbance (O.D. value) is measured by a microplate reader. The concentration-absorbance curve can be fitted by computer or mapping to calculate the concentration of Estrodial (E2) in human serum.
| Product Name | Human Estrodial (E2) ELISA Kit (Competitive ELISA) |
|---|---|
| SKU/Catalog Number | EK7049 |
| Description | Competitive High Sensitivity ELISA kit for Quantitative Detection of Human Estrodial (E2). 96wells/kit, with removable strips. |
| Cite This Product | Human Estrodial (E2) ELISA Kit (Competitive ELISA) (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7049) |
| Validated Species | Human |
| Application | ELISA *Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under Protein Target Info. |
| Cross Reactivity | There is no detectable cross-reactivity. |
| Pack Size | 96wells/kit, with removable strips. |
| Sensitivity | 40 pg/ml *Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive. |
|---|---|
| Assay Range | 40-1400 pg/ml *This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range. |
| Sample Type | Serum *The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it. **For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide. |
| Storage | Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Quantity | Volume | Buffers |
| Pre-coated 96-well strip microplate | 1 | 8 strips of 12 wells | Goat Anti- Rabbit Secondary Antibody, Polystyrene micro-well plate |
| Human Estrodial (E2) Standards(S0~S5) | 6 | 0.5ml | Estrodial (E2) (0, 40, 120, 350, 700, 1400 pg/ml), 0.02M PBS, 50% detoxification serum, 0.1% Proclin-300 |
| HRP Conjugated antigen | 1 | 6ml | HRP Conjugated Human Estrodial (E2), 0.02M PBS, 2% BSA, 0.01% azophloxine, 0.1% Proclin-300 |
| Antibody | 1 | 6ml | Rabbit anti- Human Estrodial (E2) Antibody, 0.02M PBS, 2% BSA, 0.1% Proclin-300, 0.01% Evans Blue |
| Controls | 2 | 0.5ml | Estrodial (E2), 100% natural protein, 0.1% Proclin-300 |
| 20X Wash Buffer Concentrate | 1 | 15ml | 0.2M PBS containing 0.5% tween 20 |
| Color Developing Reagent A | 1 | 7ml | 11m mol/L Urea hydrogen peroxide |
| Color Developing Reagent B | 1 | 7ml | 2m mol/L 3,3'5,5’-Tetramethylbenzidine |
| Stop Solution | 1 | 7ml | 2mol/L Sulphuric acid |
| Plate Sealers | 2 | Piece |
1. Microplate Reader capable of reading absorbance at 450nm.
2. Automated plate washer (optional)
3. Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples.
4. Deionized or distilledwater.
5. 500ml graduated cylinders.
6. Test tubes for dilution.
You can check the tissue specificity below for information on selecting positive and negative control.
*if product is indicated to react with multiple species, protein info is based on the human gene.
Click the images to enlarge.
[list_product_images] | Human Estrodial (E2) ELISA Kit standard curve[/list_product_images]
