| 数量 | 现货 |
| 供应商 | 博士德生物 |
| 检测方法 | 酶联免疫双抗夹心法 |
| 应用 | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| 适应物种 | Human |
| 规格 | 96T |
| 货号 | EK1101 |
|---|---|
| 产品名称 | Human Seprase/FAP PicoKine ELISA Kit |
| 产品描述 | For quantitative detection of human Seprase in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| 免疫原 | NSO, L26-D760 |
| 交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <10pg/ml |
| 检测范围 | 62.5pg/ml-4000pg/ml |
| 基因名称 | FAP |
| 基因全名 | fibroblast activation protein, alpha |
| Uniprot ID | Q12884 |
| 指标别名 | 170 kDa melanoma membrane-bound gelatinase|FAP|Fibroblast activation protein alpha|Integral membrane serine protease|SEPR_HUMAN|Seprase |
| 背景资料 | FAP(Fibroblast Activation Protein, Alpha) also known as FAPA or SEPRASE, is an inducible cell surface glycoprotein that was originally identified in cultured fibroblasts using monoclonal antibody F19. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. The FAP gene is mapped on 2q24.2. FAP is most closely related to DPPIV and they share about 50% of their amino acids. FAP is catalytically active as a 170kD dimer and has dipeptidase and gelatinase activity. Its gelatinase activity requires a glycine in P2 position.FAP-alpha shows 48% amino acid identity with dipeptidyl peptidase IV and 30% identity with DPP4-related protein. Northern blot analysis detected a 2.8-kb FAP-alpha mRNA in fibroblasts. Depletion of FAP-expressing cells, which made up only 2% of all tumor cells in established Lewis lung carcinomas, caused rapid hypoxic necrosis of both cancer and stromal cells in immunogenic tumors by a process involving interferon-gamma and tumor necrosis factor-alpha. |
| 显色时间 | 20-25 |
| SKU | 名称 | 数量 |
| Catalog number | Description | Quantity |
| EK1101-CAP | 96-well plate precoated with anti- human Seprase antibody | 1 |
| EK1101-ST | Lyophilized recombinant human Seprase standard | 10ng/tube×2 |
| EK1101-DA | Biotinylated anti- human Seprase antibody | 130μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 130μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 179 | 783 | 1188 | 189 | 736 | 1293 |
| Standard deviation | 8.59 | 43.84 | 80.78 | 11.52 | 49.31 | 96.97 |
| CV(%) | 4.8% | 5.6% | 6.8% | 6.1% | 6.7% | 7.5% |
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.062 | 0.132 | 0.162 | 0.268 | 0.353 | 0.737 | 1.157 | 1.844 |
