存储温度:The lyophilized powder is stable for at least 4 weeks at room temperature. It is recommended that the lyophilized and resuspended siRNAs are stored at or below -20oC. After resuspension, siRNA stock solutions ≥2 uM can undergo up to 50 freeze-thaw cycles without significant degradation. For long-term storage, it is recommended that the siRNA is stored at -70oC. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
存储溶液:The siRNAs are supplied as a lyophilized powder and shipped at room temperature.
参考文献:1. Prasad, C. K. et.al: Mos 3-prime UTR regulatory differences underlie species-specific temporal patterns of Mos mRNA cytoplasmic polyadenylation and translational recruitment during oocyte maturation. Molec. Reprod. Dev. 75: 1258-1268, 2008. 2. Mendez, R. et.al: Phosphorylation of CPE binding factor by Eg2 regulates translation of c-mos mRNA. Nature 404: 302-307, 2000.
简单描述:MOS is a pool of three individual synthetic siRNA duplexes designed to knock-down human MOS mRNA expression. Each siRNA is 19-25 bases in length.
Description: MOS is a pool of three individual synthetic siRNA duplexes designed to knock-down human MOS mRNA expression. Each siRNA is 19-25 bases in length.
Reconstitution Protocol: Briefly centrifuge the tubes (maximum RCF 4,000g) to collect lyophilized siRNA at the bottom of the tube. Resuspend the siRNA in 50 ul of DEPC-treated water (supplied by researcher), which results in a 1x stock solution (10 uM). Gently pipet the solution 3-5 times to mix and avoid the introduction of bubbles. Optional: aliquot 1x stock solutions for storage.