活性: Measured by its ability to bind Human CD32a (R131),His Tag (Catalog # CDA-H5221) in a functional ELISA.
产品背景: Immunoglobulin G2 (IgG2) is a member of many immunoglobulin G developed and secreted by effective B cells. In wake of cutting by pepsin, IgG is divided into two F(ab)s with one antigen binding site and a high conserved Fc segment. The Fc segment bears a highly conserved N-glycosylation site. There are two members of IgG2: IgG2a and IgG2b. It was found that IgG2a was superior to IgG1 in activating complement. The glycosylation of the circulating immunoglobulin-γ (IgG) antibody molecules changes in rheumatoid arthritis. Ig gamma-2 chain Fc region contains two constant regions of IgG2 H chain (CH2, CH3).
产品描述: Human IgG2 Fc, Tag Free contains no "tag", and has a calculated MW of 25.7 kDa. The predicted N-terminus is Glu 99. As a result of glycosylation, the reducing (R) protein migrates as 33-35 kDa, and the non-reducing (NR) protein migrates as 50-55 kDa in SDS-PAGE.
