Anti-Human IgG1 Fc Antibody

IgG1 antibody | AbD27686

Human anti Human IgG1 (Fc)

Anti-Human IgG1 Fc Antibody recognizes human IgG1 and can be used for the detection of therapeutic antibodies in human IgG1 format in samples obtained from cynomolgus macaques.

Product Type

Monoclonal Antibody

Clone

AbD27686

Isotype

HuCAL Fab monovalent

Human Anti-Human IgG1 Fc Antibody clone AbD27686 is a recombinant monovalent Fab antibody, that recognizes human IgG1 Fc, with an affinity of 76 nM. It has been shown to have a greatly reduced affinity (89-fold) for IgG from cynomolgus macaque compared to human IgG1. It also has strongly reduced binding to rhesus macaque IgG. It does not recognize other human immunoglobulin isotypes (IgG2, IgG3, IgG4, IgA, IgE, IgM) or mouse IgG2a.

This antibody has been generated by Bio-Rad using the HuCAL® antibody library and CysDisplay® technology. It is sold for research use, with implicit permission for use for the detection of therapeutic antibodies from samples obtained from experimental animals. When tested by Bio-Rad in an antigen capture pharmacokinetic assay format, this antibody performs similarly to Mouse Anti-Human IgG (CH2 Domain) Antibody clone R10Z8E9 (MCA5748G) in 10% cynomolgus macaque serum. It recognizes a different epitope on human IgG1 Fc than clone R10Z8E9.

Target Species

Human

Product Form

A monovalent human recombinant Fab (lambda light chain) selected from the HuCAL PLATINUM^® phage display library. Expressed in E. coli and purified using purified using NiNTA affinity chromatography. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as a liquid

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.01% Thiomersal

Immunogen

Recombinant human IgG1 kappa

Affinity

The monovalent intrinsic affinity of this antibody was measured as K_D=76 nM by real time, label free molecular interaction analysis on immobilized hIgG1 kappa

Approx. Protein Concentrations

Antibody concentration 0.5 mg/ml

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