中文：ProteoExtract@ 天然膜蛋白抽提试剂盒 (M-PEK) 
英文名称：ProteoExtract® Native Membrane Protein Extraction Kit

Description
Overview	A convenient kit for the isolation of native integral membrane and membrane-associated proteins, without the need for ultracentrifugation. Extraction is based on association of proteins with cellular membranes rather than on their hydrophobicity. Resulting samples are suitable for use in functional assays, 2-D gel electrophoresis, and other applications.
	Note: 1 kit is sufficient for up to 20 sample extractions.
Catalogue Number	444810
Brand Family	Calbiochem®
Synonyms	M-PEK Kit
Materials Required but Not Delivered	• Platform mixer e.g., IKA Vibramax (when extracting adherent tissue culture cells) • Cell culture equipment, media etc. for cell growth (e. g., RPMI, DMEM). • Micropipettes and tips, 10 µl, 200 µl and 1 ml size (e.g., Eppendorf, Gilson or equivalent) • Cooled centrifuge and rotor for 50 or 15 ml tube size (Eppendorf, Heraeus, Nalgene, etc.) • Cooled micro centrifuge and rotor up to 18,000 x g for 2 ml tube size (e.g. Eppendorf) • Thermo mixer or rolling facility (e.g., Eppendorf) • Homogenizer (e.g. potter or Dounce) or mortar.

Product Information
Form	20 Extractions
Kit contains	Wash Buffer, Extraction Buffer 1, Extraction Buffer 2, Protease Inhibitor Cocktail, and a user protocol.

Biological Information
Sample Type	Mammalian tissues and cultured cells

Product Usage Statements
Intended use	The ProteoExtract™ Native Membrane Protein Extraction Kit is designed for the isolation of native membrane proteins from a broad range of mammalian samples, including: • Adherent tissue culture cells • Suspension grown tissue culture cells • Frozen cell pellets • Tissues

Storage and Shipping Information
Ship Code	Shipped at Ambient Temperature or with Blue Ice
Toxicity	Multiple Toxicity Values, refer to MSDS
Storage	+2°C to +8°C
Storage Conditions	• Wash Buffer Store at 4°C. • Extraction Buffers I & II The Extraction Buffers I and II can be stored at 4°C for up to 6 month. For prolonged storage, freeze the buffers in convenient aliquots at -20°C. Before extraction, buffers must be thawed at room temperature (RT). After thawing, mix components by gently shaking or vortexing. Avoid repeated freezing and thawing! • Protease Inhibitor Cocktail The Protease Inhibitor Cocktail is supplied in DMSO and can be stored at 4°C up to 6 months. For prolonged storage, freeze the cocktail in convenient aliquots at -20°C. During the sample preparation procedure it must be kept at RT to prevent freezing of DMSO.
Do not freeze	Ok to freeze

ProteoExtract^@ 天然膜蛋白抽提试剂盒专门用于从哺乳动物细胞和组织中快速高效地分离天然膜蛋白。全新的M-PEK试剂盒主要从哺乳动物样本的细胞膜结构中分离膜蛋白，而不仅仅是根据蛋白的疏水性这一特性来完成膜蛋白的分离。整个操作过程无需超高速离心，也无需在较高温度下孵育样本。极为温和的反应条件可以让您的膜蛋白及膜相关蛋白在其自然状态下被完整地分离出来。由于试剂盒提供极为温和的反应条件，故而膜蛋白可以在具有天然活性状态的情况下被分离出来，分离后的膜蛋白可用于酶活分析（包括激酶活性分析）、（非变性）胶电泳、免疫印迹及分析、其他分析，如：ELISA等.膜蛋白翻译后修饰分析，如：磷酸化、SELDI、膜蛋白的芯片检测.本试剂盒提供了膜蛋白抽提所需的所有试剂，包括抽提及洗脱缓冲液、蛋白酶抑制剂等。每个试剂盒可用于20次贴壁细胞、悬浮细胞及组织等样本（2-5X10⁶个细胞或25-50mg组织每个样本）处理。
特点：
3-5倍富集天然膜蛋白
得到天然、有活性的蛋白
两步操作，仅需1至1.5小时
可平行处理多个样本
无需超高速离心