ON ELISA KIT|Human

Catalog  EF007025

Packing 48T/96T

Reactivity Human

Detect Range 1.563-100ng/ml

ON ELISA KIT Sensitivity< 0.938ng/ml

Application ON ELISA KIT|Human is for detecting the concentration of Human ON in serum, plasma, tissue homogenates and other biological fluids.

Storage condition 6 month at 4 Centigrade

Principle of ON ELISA KIT|Human

The microtiter plate provided in ON ELISA KIT|Human has been pre-coated with an Human ON antibody specific to Human ON. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated Human ONantibody preparation specific for Human ON then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Human ON, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm . The concentration of Human ONin the samples is then determined by comparing the O.D. of the samples to the standard curve.

*Please noted that ON ELISA KIT|Human is only for RUO (Research Use Only), Not for the diagnostic Purpose!