Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Lysate Type:
Tissue Lysate
Species:
Rat
Quality Assurance:
Protein concentration from the sample was measured by taking absorbency at 280 nm using BSA. Sample was run on a 4-20% polyacrylamide gel. After electrophoresis the gel was stained with coomassie blue and examined for sharp, well resolved protein bands. There was no smeariness indicating no protein degradation. The protein was transferred to PVDF membrane and further tested for the separation and integrity of proteins by using an actin antibody.
Source:
Rat
Presentation:
The lysates with dye are in Laemmli buffer with the following components : Tris Base: 12.11g/lL {0.1M, pH 6.8}
SDS :4% (w/v),{40.0g/L}, Bromophenol Blue 0.2%(w/v) {2.0g/L}, Glycerol 20% (v/v), {200ml/L in molecular biology grade water}.
Format:
Purified
Storage Conditions:
Maintain at -70°C in undiluted aliquots for up to six months after receipt. Avoid repeated freeze/thaw cycles.
Reagent Type:
Western Blotting Reagents
Concentration:
3.75μg/μL
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详细描述见链接：http://www.millipore.com/catalogue/item/CL108
